Rapid induction of morphological changes in human carcinoma cells A-431 by epidermal growth factors
Rapid induction of morphological changes in human carcinoma cells A-431 by epidermal growth factors
复制标题
表皮生长因子快速诱导人癌细胞A-431形态变化
作者：
Michael Chinkers;J. Mckanna;S. Cohen
用扫描电子显微镜观察了表皮生长因子(EGF)对人癌细胞A-431的形态影响。这些扁平的多边形细胞通常只表现出小的膜折叠，但在37℃暴露于EGF的5分钟内显示出广泛的褶皱和丝状足的伸展。这种褶皱活动是瞬时的，在另一5-15分钟内消退，但随之而来的是表面形态的其他几个变化。在接触激素的第一个小时内，细胞表面变得非常光滑，细胞核似乎突出到原本很薄的单层的平面之上，使细胞看起来像是一个煎蛋。集落边缘的细胞逐渐从底物上收回，导致单层在12小时前重组为多层集落。因此，EGF诱导这些表皮样细胞的形态发生快速和长期的变化。
The morphological effects of epidermal growth factor (EGF) on human carcinoma cells A-431 have been examined by scanning electron microscopy. These flat polygonal cells normally exhibit only small membrane folds, but show extensive ruffling and extension of filopodia within 5 min of exposure to EGF at 37 degrees C. This ruffling activity is transient, subsiding within another 5--15 min, but several other changes in surface morphology follow. Within the first hour of exposure to the hormone, the cell surface becomes exceedingly smooth and the nuclei seem to protrude above the plane of the otherwise thin monolayer, giving the cells a "fried egg" appearance. Cells at the edges of colonies gradually retract from the substrate, leading to reorganization, by 12 h, of the monolayer into multilayered colonies. EGF thus induces both rapid and long-term alterations in the morphology of these epidermoid cells.