Fluorometric determination of sulrite and nitrite in aqueous samples using a novel detection unit of a microfluidic device
Fluorometric determination of sulrite and nitrite in aqueous samples using a novel detection unit of a microfluidic device
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DOI：
10.2116/analsci.20.209
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发表时间：
2004-01-01
影响因子：
1.6
通讯作者：
Okubo, A
中科院分区：
文献类型：
--
作者：
Fujii, S;Tokuyama, T;Okubo, A
研究了N-(9-吖啶基)马来酰亚胺(NAM)和2，3-二氨基萘(DAN)在芯片上荧光检测亚硫酸盐和亚硝酸根的方法。通常，在微芯片分析中，由于发射的荧光波长不同，这些荧光试剂需要分别衍生化和检测。所提出的荧光检测单元采用不带滤光片的光纤，即使在微芯片中也可以灵敏地检测到多个波长的荧光。用聚合物芯片分析系统同时测定了环境样品中的亚硫酸盐和亚硝酸盐。亚硫酸盐和亚硝酸盐的校正曲线呈线性关系(R2=0.998(亚硫酸盐)和R2=0.990(亚硝酸盐))，4次测定的相对标准偏差分别为2.1%(亚硫酸盐20微米)和1.3%(亚硝酸盐20微米)。方法用于环境样品中亚硫酸盐和亚硝酸盐的回收试验。
On-chip fluorescence determination of sulfite and nitrite with N-(9-acridinyl)maleimide (NAM) and 2,3-diaminonaphthalene (DAN) has been developed using a novel fluorescence detection unit for microchip analysis. Usually, these fluorescence reagents are derivatized and detected separately in microchip analysis because different fluorescence wavelengths are emitted. The proposed fluorescence detection unit has optical fibers with no optical filter, and plural wavelengths of fluorescence were detected sensitively, even in the microchip. In this study, the simultaneous determination of sulfite and nitrite in environmental samples was performed with a polymer microchip analysis system. The calibration curves of sulfite and nitrite showed linear relations (R-2 = 0.998 (sulfite) and R-2 = 0.990 (nitrite)), and the relative standard deviations (RSD) for 4 runs were 2.1% (20 muM sulfite) and 1.3% (20 muM nitrite), respectively. The proposed method was applied to the recovery test of sulfite and nitrite in environmental samples.