Efficient and dispersed local P element transposition from Drosophila females.
Efficient and dispersed local P element transposition from Drosophila females.
复制标题
来自雌性果蝇的高效且分散的局部 P 元素转位。
DOI：
10.1093/genetics/133.2.361
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发表时间：
1993
期刊：
影响因子：
3.3
通讯作者：
Spradling,AC
中科院分区：
文献类型：
--
作者：
Zhang,P;Spradling,AC
我们研究了果蝇 P 元件插入是如何分布在其起始位点附近的染色体区域的。与 delta 2-3 (99B) 菌株杂交后，驻留在微型染色体 Dp1187 常染色质中的单个 P 元件被动员，通过对后代进行 Southern 印迹，以最小的偏差鉴定携带转座的后代。所有子代微型染色体中大约 1-2% 包含新插入。许多“局部换位”非常接近或位于起始 P 元素内；然而，近 1% 的子代染色体包含距供体元件 1-180 kb 的新插入。与父本相比，在雌性后代中观察到更多的局部插入，并且大多数发生在相对于起始元素的首选方向。这些观察结果表明，供体元件经常被切除并在局部重新插入，而从未从转座复合物中解离。从雌性中恢复的局部转座的高频率和多样化分布表明，通过使用位于目标靶标附近的起始 P 元件可以显着提高插入诱变的效率。
We have investigated how Drosophila P element insertions are distributed in the chromosomal region near their starting site. A single P element residing in the euchromatin of minichromosome Dp1187 was mobilized following a cross to the delta 2-3 (99B) strain, and progeny bearing transpositions were identified with a minimum of bias by performing Southern blots on progeny. Approximately 1-2% of all progeny minichromosomes contained new insertions. Many of these "local transpositions" landed very close to or within the starting P element; however, nearly 1% of all progeny chromosomes contained new insertions 1-180 kb from the donor element. More local insertions were observed in the progeny of females than from male parents, and most occurred in a preferred orientation relative to the starting element. These observations suggested that donor elements are frequently excised and reinserted locally without ever dissociating from a transposition complex. The high frequency and diverse distribution of local transpositions recovered from females suggested that the efficiency of insertional mutagenesis can be significantly enhanced by using a starting P element(s) located near the target of interest.