Autophagy is induced under Zn limitation and contributes to Zn-limited stress tolerance in Arabidopsis (Arabidopsis thaliana)
Autophagy is induced under Zn limitation and contributes to Zn-limited stress tolerance in Arabidopsis (Arabidopsis thaliana)
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DOI：
10.1080/00380768.2017.1360750
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发表时间：
2017-01-01
影响因子：
2
通讯作者：
Ishida, Hiroyuki
中科院分区：
文献类型：
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作者：
Eguchi, Masatake;Kimura, Kazuhiko;Ishida, Hiroyuki
自噬是真核生物中保守的细胞成分的降解系统。在拟南芥中，已知在黑暗诱导的碳饥饿和缺氮条件下，自噬对生长至关重要。然而，人们对自噬和其他营养物质之间的关系知之甚少。在这里，我们重点讨论自噬与锌营养之间的关系。我们发现，自噬缺陷(ATG)突变体在锌限制条件下表现出较早的衰老表型，在锌限制条件下生长恢复有限。此外，我们通过自噬相关基因(ATGs)的表达分析和含有绿色荧光蛋白-Atg8a(GFP-Atg8a)的自噬小体的成像分析，证实了锌限制下自噬的诱导。在ATG突变体中，虽然锌浓度与野生型植物相似，但缺锌诱导基因的转录水平波动更大，O-2(-)和H_2O_2水平比野生型植物更高。这些结果表明，自噬参与了细胞内锌的利用，并抑制了锌限制产生的活性氧(ROS)的积累。
Autophagy is a degradation system for cellular components conserved in eukaryotes. In Arabidopsis, it is known that autophagy is crucial for growth under dark-induced carbon starvation and N deficiency. However, little is known about the relationship between autophagy and other nutrients. Here, we focused on the relationship between autophagy and Zn nutrition. We found that autophagy-deficient (atg) mutants showed an early senescence phenotype under Zn limitation and limited growth recovery from Zn limitation. Furthermore, we confirmed the induction of autophagy under Zn limitation by expression analysis of autophagy-related genes (ATGs) and imaging analysis of autophagic bodies with green fluorescent protein-ATG8a (GFP-ATG8a). In atg mutants, although the Zn concentrations were similar to those of the wild-type plants, the transcript levels of Zn deficiency-inducible genes fluctuated more, and O-2(-) and H2O2 levels increased more than in wild-type plants. These results suggest that autophagy is involved in intracellular Zn usage and suppresses the accumulation of reactive oxygen species (ROS) generated by Zn limitation.