Bio-fluorometric realtime gas-imaging system (sniff-cam) for skin ethanol vapor
Bio-fluorometric realtime gas-imaging system (sniff-cam) for skin ethanol vapor
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DOI：
10.1109/isoen.2019.8823482
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发表时间：
2019-05
期刊：
影响因子：
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通讯作者：
Kenta Iitani;Toshiyuki Sato;Koji Toma;T. Arakawa;K. Mitsubayashi
中科院分区：
文献类型：
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作者：
Kenta Iitani;Toshiyuki Sato;Koji Toma;T. Arakawa;K. Mitsubayashi
我们开发了气体成像系统（sniff-cam），可以对气态乙醇（EtOH）进行成像。在EtOH嗅探器中，使用烟酰胺腺嘌呤二核苷酸（NAD）依赖性醇脱氢酶（ADH）。当将气态EtOH施加到具有NAD+溶液的ADH固定化网时，通过ADH介导的反应产生NADH。通过在340 nm处的UV激发，NADH在490 nm波长处发射荧光;因此，通过测量来自ADH固定的网表面上的NADH的荧光强度的分布来可视化EtOH的浓度分布。EtOH嗅探-凸轮的动态范围为0.1-1000 ppm。此外，观察到以气体出口为中心的圆形荧光梯度。结果表明，乙醇嗅探摄像机可以用于研究皮肤释放乙醇的时空变化。
We developed gas-imaging system (sniff-cam) that allowed imaging of gaseous ethanol (EtOH). In the EtOH sniff-cam, a nicotinamide adenine dinucleotide (NAD)-dependent alcohol dehydrogenase (ADH) was used. When applying gaseous EtOH to an ADH-immobilized mesh with NAD+ solution, NADH was produced through an ADH-mediated reaction. NADH emits fluorescence at wavelength of 490 nm by UV excitation at 340 nm; thus concentration distribution of EtOH was visualized by measuring the distribution of fluorescence light intensity from NADH on the ADH-immobilized mesh surface. The dynamic range of EtOH sniff-cam was 0.1–1000 ppm. Besides, rounded fluorescence gradient centered in the gas outlet was observed. Results suggested that the EtOH sniff-cam could be applied to investigate the spatiotemporal change of EtOH emanated from the skin.