Enhanced productivity of G1 phase Chinese hamster ovary cells using the GADD153 promoter
Enhanced productivity of G1 phase Chinese hamster ovary cells using the GADD153 promoter
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DOI：
10.1023/b:bile.0000009462.10772.a4
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发表时间：
2004-01-01
影响因子：
2.7
通讯作者：
Sunstrom, NA
中科院分区：
文献类型：
--
作者：
de Boer, L;Gray, PP;Sunstrom, NA
在中国仓鼠卵巢细胞中，在不同的细胞周期阶段和两种不同的生长条件下，检查了三种不同的启动子的生产力。在生长停滞和DNA损伤诱导型GADD 153启动子下，增强型绿色荧光蛋白（d2 EGFP）和分泌型碱性磷酸酶（SEAP）的短半衰期变体的生产率在细胞周期的G1期和血清饥饿时最高，而在巨细胞病毒（CMV）或猿猴病毒SV 40启动子下，生产率在S期和完全培养基中最高。这些结果表明GADD 153启动子在无蛋白条件下用于生产目的的效用。
Productivity of three different promoters at various cell cycle stages and under two distinct growth conditions was examined in Chinese hamster ovary cells. Under the Growth Arrest and DNA Damage inducible GADD153 promoter, productivity of the short half-live variant of the enhanced green fluorescent protein (d2EGFP) and the secreted alkaline phosphatase (SEAP) was highest at the G1 phase of the cell cycle and at serum starvation, while under the cytomegalovirus (CMV) or the simian virus SV40 promoter, productivity was highest at S-phase and in complete medium. These results indicate the utility of the GADD153 promoter for production purposes under protein-free conditions.