Isolation and whole-genome sequencing of a novel aviadenovirus from owls in Japan
Isolation and whole-genome sequencing of a novel aviadenovirus from owls in Japan
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DOI：
10.1007/s00705-022-05380-3
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发表时间：
2022-02
影响因子：
2.7
通讯作者：
H. Kobayashi;Y. Uchida;Kan Fujino;Masayuki Horie;Eisuke Umezawa;N. Aihara;J. Kamiie;H. Shimoda-H.-Shimod
中科院分区：
文献类型：
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作者：
H. Kobayashi;Y. Uchida;Kan Fujino;Masayuki Horie;Eisuke Umezawa;N. Aihara;J. Kamiie;H. Shimoda-H.-Shimod
据报道，腺病毒可感染多种鸟类。在这里，我们分离出一种新的腺病毒从一个死亡的猫头鹰小鸡（孟加拉鹰猫头鹰;Bubo bengalensis）在日本的猛禽养殖设施的肝脏，并确定了完整的基因组序列的病毒。我们对死亡的猫头鹰幼雏进行了尸检，发现它们的肝脏肿大，心包水肿，肝组织局灶性坏死。肝组织的透射电子显微镜显示病毒样结构，在细胞核中呈准晶体阵列，抗腺病毒抗体的免疫组化染色显示肝细胞和其他细胞呈阳性反应。试图从死亡猫头鹰小鸡的匀浆肝组织中分离病毒，结果显示在多次盲传后对鸡源培养细胞有致细胞病变作用。进一步测定了该病毒的全基因组序列，并进行了系统发育分析，结果表明该病毒属于禽腺病毒属，与鸡和火鸡的禽腺病毒形成一个簇。该病毒的DNA聚合酶与其最接近的已知腺病毒亲戚之间的氨基酸序列差异约为29%，这意味着该病毒可以被分配到禽腺病毒属中的一个新物种。根据我们的数据，这种新的猫头鹰腺病毒可能是猫头鹰致命感染的原因，这可能威胁到野生和圈养的猫头鹰种群。此外，这种病毒是独特的猛禽腺病毒，因为它感染鸡源培养细胞，提高了进一步调查，以评估这种病毒的种间传播的重要性。
Adenoviruses have been reported to infect a variety of birds. Here, we isolated a novel adenovirus from the liver of a dead owl chick (Bengal eagle owl;Bubo bengalensis) at a raptor-breeding facility in Japan and determined the complete genome sequence of the virus. We performed necropsies on the dead owl chicks and found that they had enlarged livers, pericardial edema, and focal necrosis of the liver tissue. Transmission electron microscopy of the liver tissue revealed a virus-like structure, appearing as paracrystalline arrays in the nucleus, and immunohistochemical staining with anti-adenovirus antibodies showed positive reactions in hepatocytes and other cells. Attempts to isolate the virus from homogenized liver tissue of a dead owl chick showed a cytopathic effect on chicken-derived cultured cells after multiple blind passages. Further, we determined the complete genome sequence of this virus and performed phylogenetic analysis, revealing that this adenovirus belongs to the genusAviadenovirus, forming a cluster with fowl and turkey aviadenoviruses. The amino acid sequence divergence between the DNA polymerase of this virus and its closest known adenovirus relative is approximately 29%, implying that this virus can be assigned to a new species in the genusAviadenovirus. Based on our data, this novel owl adenovirus is a likely cause of fatal infections in owls, which may threaten wild and captive owl populations. Further, this virus is unique among raptor adenoviruses in that it infects chicken-derived cultured cells, raising the importance of further investigations to evaluate interspecies transmission of this virus.