A role for the AKT1 potassium channel in plant nutrition
A role for the AKT1 potassium channel in plant nutrition
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DOI：
10.1126/science.280.5365.918
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发表时间：
1998-05-08
期刊：
影响因子：
56.9
通讯作者：
Sussman, MR
中科院分区：
文献类型：
--
作者：
Hirsch, RE;Lewis, BD;Sussman, MR
在植物中，钾作为植物生长调节剂和电荷载体发挥着重要作用。其吸收的根发生不明确的机制。为了确定钾离子通道在植物中的作用，我们进行了反向遗传筛选，并确定了一个拟南芥突变体，其中AKT 1通道基因被破坏。这种突变体的根缺乏内向整流钾通道，并显示减少钾（铷-86）的吸收。与野生型相比，突变体植株在钾浓度为100微摩尔或更低的培养基上生长不良，这些结果和膜电位测量表明，AKT 1通道介导从含有低至10微摩尔钾的溶液中吸收钾。
In plants, potassium serves an essential role as an osmoticum and charge carrier. Its uptake by roots occurs by poorly defined mechanisms. To determine the role of potassium channels in planta, we performed a reverse genetic screen and identified an Arabidopsis thaliana mutant in which the AKT1 channel gene was disrupted. Roots of this mutant lacked inward-rectifying potassium channels and displayed reduced potassium (rubidium-86) uptake. Compared with wild type, mutant plants grew poorly on media with a potassium concentration of 100 micromolar or less. These results and membrane potential measurements suggest that the AKT1 channel mediates potassium uptake from solutions that contain as little as 10 micromolar potassium.