Dual Fluorescence and NMR Study for the Interaction between Xanthene Dyes and Nanoparticles
Dual Fluorescence and NMR Study for the Interaction between Xanthene Dyes and Nanoparticles
复制标题
呫吨染料与纳米粒子相互作用的双荧光和核磁共振研究
DOI：
10.1021/acs.langmuir.0c03020
复制
发表时间：
2021
期刊：
影响因子：
3.9
通讯作者：
Casabianca, Leah Beck
中科院分区：
文献类型：
--
作者：
Xu, Hui;Casabianca, Leah Beck
荧光染料和纳米颗粒（NP）利用其独特的特性被广泛一起用于制造新型生物传感器。拥有使我们能够获得有关纳米粒子和荧光染料之间相互作用的详细和高分辨率信息的技术至关重要。在这项工作中，我们选择罗丹明 B (RhB) 和脒改性聚苯乙烯 (CML) 纳米粒子作为模型，并采用 NMR（1H 和 STD-NMR）和光学（紫外可见分光光度计和荧光）技术来研究 NP 和荧光染料之间的相互作用。从紫外可见分光光度计和荧光光谱中，我们发现当罗丹明 B 与羧酸盐改性的聚苯乙烯纳米颗粒结合时，比与脒改性的纳米颗粒结合时有更大的红移。相应地，RhB 与 CML NP 结合时比脒 NP 具有更宽的 NMR 峰和更大的 STD 效应。这两种技术的结果相互验证。值得注意的是，核磁共振技术比紫外-可见光和荧光方法提供更可靠的数据。此外，我们表明核磁共振技术，特别是 STD-NMR，可以提供更多原子级的结合几何信息。 RhB 较小芳环的较高 STD 效应表明，当与聚苯乙烯纳米粒子结合时，该芳环更接近纳米粒子表面。
Fluorescent dyes and nanoparticles (NPs) have been widely used together to make novel biosensors, taking advantage of their unique characteristics. It is crucial to have techniques that enable us to gain detailed and high-resolution information regarding the interaction between NPs and fluorescent dyes. In this work, we chose rhodamine B (RhB) and amidine- and carboxylate-modified polystyrene (CML) NPs as models and employed both NMR (1H and STD-NMR) and optical (UV–vis and fluorescence) techniques to investigate the interaction between NPs and fluorescent dyes. From UV–vis and fluorescence spectroscopy, we see that there are larger red shifts when rhodamine B binds to carboxylate-modified polystyrene NPs than amidine-modified NPs. Correspondingly, RhB has broader NMR peaks and a larger STD effect when binding to CML NPs than amidine NPs. Results from these two techniques validate each other. It is notable that the NMR techniques provide more reliable data than UV–vis and fluorescence methods. Moreover, we show that NMR techniques, especially STD-NMR, can provide more atomic-level binding geometry information. The higher STD effect of the smaller aromatic ring of RhB implies that this aromatic ring is closer to the surface of NPs when binding to polystyrene NPs.