Modeling DNA shuffling.
Modeling DNA shuffling.
复制标题
DNA 改组建模。
DOI：
10.1089/cmb.1999.6.77
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Sun,F
中科院分区：
文献类型：
--
作者：
Sun,F
体外进化是一种新的、重要的实验室方法（在V&V中），用于进化具有所需性质的分子。它已被用于各种生物学研究和药物开发。本文研究了体外进化实验中一种重要的实验方法--DNA shuB。凌我们建立了一个DNA穿梭的数学模型。研究了基于该模型的DNA和ufEng实验的进展。该模型由两部分组成。首先，我们应用Lander-Waterman模型进行物理映射，通过对随机克隆进行指纹识别来模拟可以通过DNA改组重新组装的区域的分布。然后，我们提出了一个模型，不同的DNA物种之间的重组与diikent突变。我们将我们的理论结果与实验结果进行比较，发现理论预测与实验结果完全吻合。最后，我们提出了新的应用的理论结果的物理映射使用DNA舒。iBing。该模型可用于DNA改组实验的设计和利用DNA改组作为诱变技术的体外进化实验的设计。
In vitm evolution is anew, important laboratory method (in V&V) to evolve molecules with desired properties. It has been used in a variety of biological studies and drug development. Iu this paper, we study one important experimeutd method used in in vitro evolution experiments called DNA shuB. ling. We construct a mathematical model for DNA shufEl. ing and study the progress of DNA &ufEng experiments based on this model. The model consists of two parts. First we apply the Lander-Waterman model for physical mapping by fingerprinting random clones to model the distribution of regions that can be reassembled through DNA shuflling. Then we present a model for recombination between different DNA species with diikent mutations. We compare our theoretical results with experimental results and find perfect fit for the theoretical predictions with the experimental results. Finally we propose novel applications of the theoretical results to physical mapping using DNA shu. iBing. This model should be useful for the design of DNA shui% iug experiments and the design of in vitro evolution experiments using DNA shuffling as mutagenesis techniques.