Cellular and metabolic approaches to investigate the effects of graphene and graphene oxide in the fungi Aspergillus flavus and Aspergillus niger
Cellular and metabolic approaches to investigate the effects of graphene and graphene oxide in the fungi Aspergillus flavus and Aspergillus niger
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DOI：
10.1016/j.carbon.2018.10.099
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发表时间：
2019-03-01
期刊：
影响因子：
10.9
通讯作者：
Rodrigues, Debora E.
中科院分区：
文献类型：
--
作者：
Hang Ngoc Nguyen;Chaves-Lopez, Clemencia;Rodrigues, Debora E.
结合挥发性有机化合物(VOC)代谢组学、酶活性和形态研究等方法，对碳基纳米材料和真菌之间的毒性相互作用提供了深入的了解。研究的模式真菌为黑曲霉和黄曲霉。他们暴露在石墨烯(G)和氧化石墨烯(GO)中，生物量和异常菌丝方面减少了62%。这些微生物在这些纳米材料存在下的应激反应进一步取决于通过细胞凋亡分析和真菌菌丝产生的活性氧(ROS)而发生的整体细胞变化。暴露在纳米材料下的这些微生物产生的ROS证实了细胞的氧化应激，这解释了在纳米材料存在下观察到的凋亡样细胞死亡。这种真菌反应还与几种重要的酶的产生减少有关，这些酶参与细胞生长所需营养物质的分解代谢，如酸性磷酸酶、萘酚-ASBI磷酸水解酶、β-葡萄糖苷酶和β-半乳糖苷酶。这些纳米材料的存在也引发了真菌产生VOC的变化。这些变化表明应激条件，因为这些化合物在真菌新陈代谢中具有重要作用。总体而言，黑曲霉对GO更敏感，黄曲霉对G.(C)2018爱思唯尔有限公司更敏感。保留所有权利。
A combination of approaches, including volatile organic compound (VOC) metabolomics, enzymatic activity and morphological investigations, are employed to provide insights on the toxic interactions among carbon-based nanomaterials and fungi. The model fungi investigated are Aspergillus niger and Aspergillus flavus. Their exposure to graphene (G) and graphene oxide (GO) presents 62% reduction in biomass and abnormal hyphae aspect. The stress response of these microorganisms in the presence of these nanomaterials is further determined by overall cell changes through apoptosis analyses and production of reactive oxygen species (ROS) by the fungal hyphae. The ROS production by these microorganisms exposed to the nanomaterials confirms cellular oxidative stress, which explains the apoptoticlike cell death observed in the presence of the nanomaterials. This fungal response is also linked to lower production of several important enzymes involved in the catabolism of nutrients for cell growth, such as acid phosphatase, Naphthol-ASBI phosphohydrolase, beta-glucosidase and beta-galactosidase. The presence of these nanomaterials also triggers changes in the production of VOC by the fungi. These changes are indicative of stress conditions since these compounds have significant roles in fungal metabolism. Overall, A. niger was more sensitive to GO and A. flavus was more sensitive to G. (C) 2018 Elsevier Ltd. All rights reserved.