Modifying preselected sites on proteins: the stretch of residues 633-642 of the myosin heavy chain is part of the actin-binding site.
Modifying preselected sites on proteins: the stretch of residues 633-642 of the myosin heavy chain is part of the actin-binding site.
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修饰蛋白质上的预选位点：肌球蛋白重链的残基 633-642 是肌动蛋白结合位点的一部分。
DOI：
10.1073/pnas.85.20.7471
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发表时间：
1988
影响因子：
11.1
通讯作者：
Morales,MF
中科院分区：
文献类型：
--
作者：
Chaussepied,P;Morales,MF
我们已经设计了一种“抗肽”，能够牢固和特异性地与肌球蛋白S-1重链的预选伸展相互作用。该抗肽与其靶向延伸段（残基633-642）的共价连接不影响蛋白质的固有ATP酶活性，但显著降低肌球蛋白头部的肌动蛋白结合能力。
We have designed an "antipeptide" capable of firmly and specifically interacting with a preselected stretch of myosin S-1 heavy chain. Covalent attachment of this antipeptide to its target stretch, residues 633-642, does not affect the intrinsic ATPase activities of the protein but significantly reduces the actin-binding capabilities of the myosin head.