GhmiR858 Inhibits the Accumulation of Proanthocyanidins by Targeting GhTT2L in Cotton (Gossypium hirsutum)
GhmiR858 Inhibits the Accumulation of Proanthocyanidins by Targeting GhTT2L in Cotton (Gossypium hirsutum)
复制标题
DOI：
10.1021/acs.jafc.3c03884
复制
发表时间：
2023-10-03
影响因子：
6.1
通讯作者：
Sun，Yuqiang
中科院分区：
文献类型：
--
作者：
Mei，Jun;Niu，Qingqing;Sun，Yuqiang
原花青素（PA）主要在转录水平上由复杂的调控网络进行调控。在棉花中，miRNAs作为转录后水平的关键调控因子的作用仍不清楚。在这里，我们证明了GhmiR858通过靶向GhTT2L负调控棉花叶片和愈伤组织中PA的积累。GhmiR858的过度表达抑制了GhTT2L的表达，导致PA丰度显著降低。相反，GhmiR858活性的降低上调了GhTT2L，这增加了PA积累。此外，GhTT2L被发现在棉花和拟南芥中正调控PA积累。进一步的分析表明GhTT2L与转录因子GhTTG1相互作用，后者直接与GhANR启动子结合，从而促进其转录。本研究为进一步研究miRNAs对多胺的调控机制以及选育富含多胺的彩色棉新品种提供了新的信息。
Proanthocyanidins (PAs) are predominantly regulated at the transcriptional level by sophisticated regulatory networks. In cotton, the role of miRNAs as key regulatory factors at the post-transcriptional level is still unclear. Here, we demonstrated that GhmiR858 negatively regulates PA accumulation in cotton leaves and calli by targetingGhTT2L. Excessive expression of GhmiR858 restrained the expression ofGhTT2L, resulting in a significant decrease in PA abundance. Conversely, a reduction in GhmiR858 activity upregulatedGhTT2L, which increased PA accumulation. Additionally, GhTT2L was found to positively regulate PA accumulation in both cotton andArabidopsis. Further analyses showed that GhTT2L interacted with transcription factor GhTTG1, which directly binds to theGhANRpromoter, to facilitate its transcription. This study provides new information to guide future studies of the PA regulatory mechanisms affected by miRNAs as well as the breeding of novel varieties of colored cotton with rich PAs.