PRP4 (RNA4) FROM SACCHAROMYCES-CEREVISIAE - ITS GENE-PRODUCT IS ASSOCIATED WITH THE U4/U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE
PRP4 (RNA4) FROM SACCHAROMYCES-CEREVISIAE - ITS GENE-PRODUCT IS ASSOCIATED WITH THE U4/U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE
复制标题
DOI：
10.1128/mcb.9.9.3698
复制
发表时间：
1989-09-01
影响因子：
5.3
通讯作者：
FRIESEN, JD
中科院分区：
文献类型：
--
作者：
BJORN, SP;SOLTYK, A;FRIESEN, JD
PRP4（RNA4）基因产物参与酵母细胞中的核mRNA加工;我们先前通过互补温度敏感性突变克隆了该基因。克隆基因的序列和转录本分析预测基因产物为52千道尔顿蛋白质，这用针对PRP4基因产物产生的抗体证实。这些抗体在体外抑制前体mRNA剪接，证明了PRP4在剪接中的直接作用。与抗体的免疫沉淀表明，PRP4蛋白与U4/U6小核核糖核蛋白颗粒。
The PRP4 (RNA4) gene product is involved in nuclear mRNA processing in yeast cells; we have previously cloned the gene by complementation of a temperature-sensitive mutation. Sequence and transcript analyses of the cloned gene predicted the gene product to be a 52-kilodalton protein, which was confirmed with antibodies raised against the PRP4 gene product. These antibodies inhibited precursor mRNA splicing in vitro, demonstrating a direct role of PRP4 in splicing. Immunoprecipitations with the antibodies indicated that the PRP4 protein is associated with the U4/U6 small nuclear ribonucleoprotein particle.