Implications of Bit1 and AIF overexpressions in esophageal squamous cell carcinoma
Implications of Bit1 and AIF overexpressions in esophageal squamous cell carcinoma
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Bit1 和 AIF 过表达在食管鳞状细胞癌中的意义
DOI：
10.1007/s13277-013-1073-8
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发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Liu, Hongtao
中科院分区：
文献类型：
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作者：
Fan, Tianli;Tian, Fang;Liu, Hongtao
大量证据表明，Bit1和AIF作为线粒体蛋白参与多种肿瘤的发生和发展。然而，它们在食管鳞状细胞癌（ESCC）中的表达和生物学功能尚不清楚。在本研究中，我们发现ESCC组织中Bit1、AIF、Bcl-2水平显著高于正常食管上皮组织和非典型增生组织（P< 0.05）。逐步调查显示，Bit1和Bcl-2水平与淋巴结转移和TNM分期密切相关（P< 0.05）， Bit1 mRNA水平以及AIF和Bcl-2蛋白水平与肿瘤分化密切相关（P< 0.05），但与患者的年龄和性别无关（P< 0.05）。重要的是，有淋巴结转移和TNM分期的ESCC III期和IV期的Bit1 mRNA和蛋白水平明显高于无淋巴结转移和TMN分期的ESCC I期和II期。进一步分析发现，Bit1蛋白的表达与AIF、Bcl-2蛋白的表达均呈正相关（r分别为0.408、0.405，P< 0.05）。正确的是，我们之前引用的数据表明Bit1在ESCC的发生和进展中起着关键作用，其在ESCC中的生物学功能可能与AIF和Bcl-2水平密切相关。
Overwhelming evidence has demonstrated that Bit1 and AIF as mitochondrial proteins are implicated in the development and progression of a variety of tumors. However, their expressions and biological functions in esophageal squamous cell carcinoma (ESCC) remain to be delineated. In the present study, we found that Bit1, AIF, and Bcl-2 levels in ESCC tissues were significantly higher than those in normal esophageal epithelial tissues and dysplasia tissues (P< 0.05). Stepwise investigation demonstrated that Bit1 and Bcl-2 levels were both tightly associated with lymphatic metastasis and TNM staging (P< 0.05), and the levels of Bit1 mRNA as well as AIF and Bcl-2 proteins were both closely related to tumor differentiation (P< 0.05), but not related to the patients' age and gender (P> 0.05). Importantly, Bit1 mRNA and protein levels in ESCC with lymphatic metastasis and TNM staging in III and IV were markedly higher than that without lymphatic metastasis and TMN staging in I and II. Further analysis showed that expression of Bit1 protein was both positively correlated with expressions of AIF and Bcl-2 proteins (r= 0.408 and 0.405, respectively;P< 0.05). Correctively, our data cited earlier suggest that Bit1 plays pivotal roles in the development and progression of ESCC, and its biological functions in ESCC may be closely associated with AIF and Bcl-2 levels.