pH required to kill Enterococcus faecalis in vitro
pH required to kill Enterococcus faecalis in vitro
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DOI：
10.1097/00004770-200404000-00008
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发表时间：
2004-04-01
影响因子：
4.2
通讯作者：
Eleazer, PD
中科院分区：
文献类型：
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作者：
McHugh, CP;Zhang, P;Eleazer, PD
粪肠球菌能耐受强碱性环境，但杀死粪肠球菌所需的pH值却很低。粪便是未知的。本研究以0.5的增量从pH 9.5至12测试生长。每组使用12个培养管。使用浊度、目视标尺和分光光度计测量阳性生长。在24 h时，在pH 9.5和10下，在所有试管中均观察到生长。在48 h时，所有pH 10.5管均显示生长。在72 h时，6个pH 11管显示生长。7天后，剩余的pH 11试管中有5个呈阳性。在任何pH 11.5或pH 12试管中均未发生生长。pH值在10.5 ~ 11.0范围内对E.粪杆菌，而没有管显示在pH 11.5或更高的生长。
Enterococcus faecalis tolerates highly alkaline environments, yet the exact pH required for killing E. faecalis is not known. This study tests growth at 0.5 increments from pH 9.5 to 12. Twelve culture tubes were used in each group. Positive growth was measured using turbidity, a visual scale, and a spectrophotometer. At 24 h, growth was observed in all tubes at pH 9.5 and 10. At 48 h, all pH 10.5 tubes showed growth. At 72 h, six of the pH 11 tubes showed growth. After 7 days, five of the remaining pH 11 tubes were positive. No growth occurred in any of the pH 11.5 or pH 12 tubes. Apparently, pH 10.5 to 11.0 retards growth of E. faecalis, whereas no tubes showed growth at pH 11.5 or greater.