Role of adenosine in noradrenergic neurotransmission.
Role of adenosine in noradrenergic neurotransmission.
复制标题
腺苷在去甲肾上腺素能神经传递中的作用。
DOI：
10.1152/ajpheart.1988.255.2.h386
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Jackson,EK
中科院分区：
文献类型：
--
作者：
Kuan,CJ;Jackson,EK
本研究的目的是评估内源性腺苷在体内持续交感神经刺激期间调节与组织血流量减少相关的去肾上腺素能神经传递的假设。通过比较动脉周围（交感）神经刺激（PNS）对对照大鼠和用腺苷受体拮抗剂1,3-二丙基-8-对巯基黄嘌呤（DPSPX； 10 mg + 150微克/分钟iv）治疗的大鼠肠系膜血流和去甲肾上腺素（NE）溢出的影响，在体内对这一假设进行了验证。在对照大鼠和经DPSPX预处理的大鼠中，持续的PNS （7 Hz, 30 min）导致肠系膜血流量最初大幅减少，NE外溢增加；然而，这些反应在30分钟的刺激期间减弱。经DPSPX治疗后，pns诱导的肠系膜血流变化和NE外溢的时间进程未发生改变。然而，给药DPSPX可以阻止2-氯腺苷对大鼠肠系膜去甲肾上腺素能神经传递的抑制，这表明DPSPX达到了有效水平。我们得出结论，即使在持续的交感神经刺激与组织灌注减少相关时，内源性腺苷也不会调节大鼠肠系膜体内的去肾上腺素能神经传递。
The purpose of this study was to evaluate the hypothesis that endogenous adenosine modulates noradrenergic neurotransmission in vivo during sustained periods of sympathetic nerve stimulation associated with a reduction in tissue blood flow. This hypothesis was tested in the rat mesentery in vivo by comparing the effects of periarterial (sympathetic) nerve stimulation (PNS) on mesenteric blood flow and norepinephrine (NE) spillover from the mesentery in control rats vs. rats treated with the adenosine receptor antagonist 1,3-dipropyl-8-p-sulfophenylxanthine (DPSPX; 10 mg + 150 micrograms/min iv). In both control rats and rats pretreated with DPSPX, sustained PNS (7 Hz for 30 min) caused an initial large decrease in mesenteric blood flow and increase in NE spillover; however, these responses attenuated over the 30-min stimulation period. The time course of PNS-induced changes in mesenteric blood flow and NE spillover were not altered by treatment with DPSPX. However, administration of DPSPX prevented inhibition of noradrenergic neurotransmission in the rat mesentery by 2-chloroadenosine, which indicated that an effective level of DPSPX was achieved. We conclude that even during sustained sympathetic nerve stimulation associated with reductions in tissue perfusion, endogenous adenosine does not modulate noradrenergic neurotransmission in vivo in the rat mesentery.