Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening.
Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening.
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酶促生成的CRISPR库用于基因组标记和筛选。
DOI：
10.1016/j.devcel.2015.06.003
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发表时间：
2015-08-10
影响因子：
11.8
通讯作者：
Heald R
中科院分区：
文献类型：
--
作者：
Lane AB;Strzelecka M;Ettinger A;Grenfell AW;Wittmann T;Heald R
基于 CRISPR 的技术已成为改变基因组和标记染色体位点的强大工具，但缺乏一种廉价的方法来生成大量用于全基因组筛选和标记的 RNA 向导。使用允许从任何DNA来源构建文库的方法，我们生成了引导文库，该文库可标记非洲爪蟾卵提取物中的重复基因座或单个染色体基因座，并表明复杂的文库可以高频率地靶向大肠杆菌基因组。
CRISPR-based technologies have emerged as powerful tools to alter genomes and mark chromosomal loci, but an inexpensive method for generating large numbers of RNA guides for whole genome screening and labeling is lacking. Using a method that permits library construction from any source of DNA, we generated guide libraries that label repetitive loci or a single chromosomal locus in Xenopus egg extracts and show that a complex library can target the E. coli genome at high frequency.