OPTICAL BIOSENSOR FOR ENVIRONMENTAL ONLINE MONITORING OF NAPHTHALENE AND SALICYLATE BIOAVAILABILITY WITH AN IMMOBILIZED BIOLUMINESCENT CATABOLIC REPORTER BACTERIUM
OPTICAL BIOSENSOR FOR ENVIRONMENTAL ONLINE MONITORING OF NAPHTHALENE AND SALICYLATE BIOAVAILABILITY WITH AN IMMOBILIZED BIOLUMINESCENT CATABOLIC REPORTER BACTERIUM
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DOI：
10.1128/aem.60.5.1487-1494.1994
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发表时间：
1994-05-01
影响因子：
4.4
通讯作者：
SAYLER, GS
中科院分区：
文献类型：
--
作者：
HEITZER, A;MALACHOWSKY, K;SAYLER, GS
基于基因工程生物发光分解代谢报告细菌的光学全细胞生物传感器的开发，用于连续在线监测废水中萘和水杨酸的生物利用度和微生物分解代谢活性的潜力。生物发光报告细菌荧光假单胞菌HK 44携带转录nahG-luxCDABE融合体，用于萘和水杨酸盐分解代谢。暴露于任一化合物导致诱导生物发光。通过使用海藻酸锶将报告培养物固定到光学光导的表面上。然后将该生物传感器探针插入同时接收废物流溶液和维持介质的测量池中。在规定的条件下暴露于萘和水杨酸盐导致生物发光迅速增加。响应的幅度和响应时间具有浓度依赖性。良好的再现性的响应过程中观察到的重复扰动与萘或水杨酸盐。暴露于其他化合物，如葡萄糖和复杂的营养培养基或甲苯，导致无论是轻微的生物发光增加后显着较长的响应时间相比，萘或无响应，分别。通过使用真实的污染物混合物测试了生物传感器的环境实用性。一个特定的生物发光响应后，暴露于JP-4喷气燃料饱和的水溶液或含水渗滤液从沼气厂土壤，因为萘是存在于这两种污染物的混合物。
An optical whole-cell biosensor based on a genetically engineered bioluminescent catabolic reporter bacterium was developed for continuous on-line monitoring of naphthalene and salicylate bioavailability and microbial catabolic activity potential in waste streams. The bioluminescent reporter bacterium, Pseudomonas fluorescens HK44, carries a transcriptional nahG-luxCDABE fusion for naphthalene and salicylate catabolism. Exposure to either compound resulted in inducible bioluminescence. The reporter culture was immobilized onto the surface of an optical light guide by using strontium alginate. This biosensor probe was then inserted into a measurement cell which simultaneously received the waste stream solution and a maintenance medium. Exposure under defined conditions to both naphthalene and salicylate resulted in a rapid increase in bioluminescence. The magnitude of the response and the response time were concentration dependent. Good reproducibility of the response was observed during repetitive perturbations with either naphthalene or salicylate. Exposure to other compounds, such as glucose and complex nutrient medium or toluene, resulted in either minor bioluminescence increases after significantly longer response times compared with naphthalene or no response, respectively. The environmental utility of the biosensor was tested by using real pollutant mixtures. A specific bioluminescence response was obtained after exposure to either an aqueous solution saturated with JP-4 jet fuel or an aqueous leachate from a manufactured-gas plant soil, since naphthalene was present in both pollutant mixtures.