Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment
Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment
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DOI：
10.1111/j.1365-294x.2007.03089.x
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发表时间：
2007-03-01
影响因子：
4.9
通讯作者：
Marshall, Tristan C.
中科院分区：
文献类型：
--
作者：
Kalinowski, Steven T.;Taper, Mark L.;Marshall, Tristan C.
基因分型经常被用来鉴定亲子关系。众所周知，这种分析很容易受到基因分型错误的影响，关于如何解决这个问题，人们正在进行辩论。许多科学家使用计算机程序Cervus来估计亲子关系，并利用它的选项来允许基因分型错误。在这项研究中，我们表明Cervus 1.0和2.0版本使用的用于适应基因分型错误的似然方程错误地计算了观察到错误基因的概率。对朗姆马鹿亲子关系的计算机模拟和重新分析表明，纠正这个错误会增加亲子关系分配的成功率，而且当出现错误时，容纳基因分型错误显然是有好处的。新版本的Cervus(3.0)实现了修正的似然方程，可在www.fieldgentics.com上找到。
Genotypes are frequently used to identify parentage. Such analysis is notoriously vulnerable to genotyping error, and there is ongoing debate regarding how to solve this problem. Many scientists have used the computer program CERVUS to estimate parentage, and have taken advantage of its option to allow for genotyping error. In this study, we show that the likelihood equations used by versions 1.0 and 2.0 of CERVUS to accommodate genotyping error miscalculate the probability of observing an erroneous genotype. Computer simulation and reanalysis of paternity in Rum red deer show that correcting this error increases success in paternity assignment, and that there is a clear benefit to accommodating genotyping errors when errors are present. A new version of CERVUS (3.0) implementing the corrected likelihood equations is available at www.fieldgenetics.com.