Recombinant Human Butyrylcholinesterase As a New-Age Bioscavenger Drug: Development of the Expression System
Recombinant Human Butyrylcholinesterase As a New-Age Bioscavenger Drug: Development of the Expression System
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DOI：
10.32607/20758251-2013-5-1-73-84
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发表时间：
2013-01-01
期刊：
影响因子：
2
通讯作者：
Ponomarenko, N. A.
中科院分区：
文献类型：
--
作者：
Ilyushin, D. G.;Haertley, O. M.;Ponomarenko, N. A.
丁酰胆碱酯酶（BChE）是一种丝氨酸水解酶（EC 3.1.1.8），可在大多数动物组织中发现。这种酶对有机磷化合物具有广谱的功效，这使其成为化学计量生物清除剂作用的主要候选者。开发一种新时代的DNA编码的生物清除剂是一项艰巨的任务。在过去的20年中，开发了几种人类BChE的转基因表达系统;然而，它们中没有一个被证明具有经济意义或被批准用于人类。在这项研究中，CHO为基础的表达系统进行了重新设计，导致功能性重组人丁酰胆碱酯酶的生产水平显着增加相比，迄今为止现有的系统。用Elman法和ELISA法对重组酶进行了鉴定。
Butyrylcholinesterase (BChE) is a serine hydrolase (EC 3.1.1.8) which can be found in most animal tissues. This enzyme has a broad spectrum of efficacy against organophosphorus compounds, which makes it a prime candidate for the role of stoichiometric bioscavenger. Development of a new-age DNA-encoded bioscavenger is a vival task. Several transgenic expression systems of human BChE were developed over the past 20 years; however, none of them has been shown to make economic sense or has been approved for administration to humans. In this study, a CHO-based expression system was redesigned, resulting in a significant increase in the production level of functional recombinant human butyrylcholinesterase as compared to the hitherto existing systems. The recombinant enzyme was characterized with Elman and ELISA methods.