Determination of carbon isotope ratios for honey samples by means of a liquid chromatography/isotope ratio mass spectrometry system coupled with a post-column pump
Determination of carbon isotope ratios for honey samples by means of a liquid chromatography/isotope ratio mass spectrometry system coupled with a post-column pump
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DOI：
10.1002/rcm.8170
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发表时间：
2018-08-15
影响因子：
2
通讯作者：
Suto, Nana
中科院分区：
文献类型：
--
作者：
Kawashima, Hiroto;Suto, Momoka;Suto, Nana
液相色谱/同位素比质谱（LC/IRMS）已被用于鉴定和跟踪产品，如蜂蜜，葡萄酒和柠檬汁，以及咖啡因和农药等化合物。然而，LC/IRMS具有几个缺点，包括CO2膜的高成本和被固化的过硫酸钠堵塞。在这里，我们开发了一种改进的系统，用于确定碳同位素比值使用LC/IRMS.MethodsThe主要的改进是使用柱后泵。使用改进的系统，我们确定了C-13值的葡萄糖具有较高的准确度和精密度（分别为0.1和0.1，n=3）。结果蜂蜜中葡萄糖、果糖、二糖、三糖和有机酸的C-13值分别为-27.0 ~-24.2 ‰，-26.8 ~-24.0 ‰，-28.8 ~-24.0 ‰，-27.8至-22.8份/千，和-30.6至-27.4份/千。的分析时间是三分之一到一半的分析所需的通过以前报道的methods.ConclusionsThe柱流速可以任意调整与柱后泵。我们将改进的方法应用于26个商业蜂蜜样品。我们的研究结果可以预期是有用的其他研究人员谁使用LC/IRMS。
RationaleLiquid chromatography/isotope ratio mass spectrometry (LC/IRMS) has been used to authenticate and trace products such as honey, wine, and lemon juice, and compounds such as caffeine and pesticides. However, LC/IRMS has several disadvantages, including the high cost of the CO2 membrane and blocking by solidified sodium persulfate. Here, we developed an improved system for determining carbon isotope ratios using LC/IRMS.MethodsThe main improvement was the use of a post-column pump. Using the improved system, we determined C-13 values for glucose with high accuracy and precision (0.1 and 0.1, respectively; n=3). The glucose, fructose, disaccharide, trisaccharide, and organic acid constituents of honey samples were analyzed using LC/IRMS.ResultsThe C-13 values for glucose, fructose, disaccharides, trisaccharides, and organic acids ranged from -27.0 to -24.2 parts per thousand, -26.8 to -24.0 parts per thousand, -28.8 to -24.0 parts per thousand, -27.8 to -22.8 parts per thousand, and-30.6 to -27.4 parts per thousand, respectively. The analysis time was a third to a half of that required for analysis by previously reported methods.ConclusionsThe column flow rate could be arbitrarily adjusted with the post-column pump. We applied the improved method to 26 commercial honey samples. Our results can be expected to be useful for other researchers who use LC/IRMS.