Evolution of the genus Leishmania as revealed by comparisons of nuclear DNA restriction fragment patterns.
Evolution of the genus Leishmania as revealed by comparisons of nuclear DNA restriction fragment patterns.
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通过比较核 DNA 限制性片段模式揭示利什曼原虫属的进化。
DOI：
10.1073/pnas.84.2.484
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发表时间：
1987
影响因子：
11.1
通讯作者：
Pratt,DM
中科院分区：
文献类型：
--
作者：
Beverley,SM;Ismach,RB;Pratt,DM
限制性核酸内切酶 DNA 片段模式已用于检查 28 个利什曼原虫分离株以及短膜虫、内锥虫和克氏锥虫之间的关系。用六种限制性酶产生核 DNA 片段，并将印迹与来自三个基因座的探针杂交。在主要谱系中，碎片模式本质上是完全不同的，而在主要谱系内部却发现了不同程度的分歧。使用 Nei 和 Li 的方法构建分子进化树，根据共享片段的比例来估计菌株之间的核苷酸序列差异百分比。确定的群体，例如主要谱系内的物种或亚种，也通过核 DNA 比较进行分组。在杜诺瓦尼复合体中，我们发现杜氏利什曼原虫恰加斯和婴儿利什曼原虫与杜诺瓦尼利什曼原虫内的菌株相似，这与其他研究人员提出的新大陆内脏利什曼病起源于最近的观点一致。
Restriction endonuclease DNA fragment patterns have been used to examine the relationships among 28 isolates of Leishmania as well as Crithidia, Endotrypanum, and Trypanosoma cruzi. Fragments of nuclear DNA were generated with six restriction enzymes, and blots were hybridized with probes from three loci. Among the major lineages the fragment patterns are essentially completely different, while within the major lineages various degrees of divergence are found. Molecular evolutionary trees were constructed using the method of Nei and Li to estimate the percent nucleotide sequence divergence among strains from the fraction of fragments shared. Defined groups, such as species or subspecies within the major lineages, are also grouped by nuclear DNA comparisons. Within the donovani complex, we find Leishmania donovani chagasi and Leishmania donovani infantum to be as similar as strains within Leishmania donovani donovani, consistent with the proposal by other workers that New World visceral leishmaniasis originated quite recently.
利什曼病。
DOI：
--
发表时间：
1974
期刊：
Progress in drug research. Fortschritte der Arzneimittelforschung. Progres des recherches pharmaceutiques
影响因子：
--
作者：
E. Steck
通讯作者：
E. Steck
DOI：
--
发表时间：
1979
期刊：
影响因子：
--
作者：
A. E. R. Taylor;R. Muller
通讯作者：
R. Muller