Insights into the hippocampus proteome and phosphorylation modification alterations in C57BL/6 revealed the memory improvement mechanisms of a walnut-derived peptide
Insights into the hippocampus proteome and phosphorylation modification alterations in C57BL/6 revealed the memory improvement mechanisms of a walnut-derived peptide
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对 C57BL/6 中海马蛋白质组和磷酸化修饰改变的深入了解揭示了核桃衍生肽的记忆改善机制
DOI：
10.1016/j.foodres.2022.111311
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发表时间：
2022-05-02
影响因子：
8.1
通讯作者：
Min, Weihong
中科院分区：
文献类型：
--
作者：
Geng, Mingxian;Zhao, Fanrui;Min, Weihong
本工作旨在探讨核桃源多肽WNP-10改善记忆的潜在机制。Morris水迷宫实验、超微结构观察、苏木精-伊红染色和尼氏染色结果表明，WNP-10能显著改善东莨菪碱损伤小鼠的学习记忆能力。四维无标记定量蛋白质组学分析发现，WNP-10治疗组与东莨菪碱诱导的损伤组相比，有88个差异表达的蛋白质。途径浓缩分析和Western blotting结果表明，WNP-10可通过调节磷脂酰肌醇-3-磷酸-5-激酶、组织蛋白酶L、N-乙酰氨基半乳糖6-硫酸酯硫酸酯酶和AP-3复合亚单位MU-1的表达来影响肌醇磷酸代谢，从而维持东莨菪碱损伤小鼠溶酶体的稳态。值得注意的是，磷酸蛋白质组学的结果表明，WNP-10的注射导致磷脂酰肌醇-3-磷酸-5-激酶的磷酸化增加。这些发现为核桃肽改善记忆的潜在机制提供了新的见解。
This work aimed to explore the underlying mechanisms of memory improvement effects of a walnut derived peptide WNP-10. The morris water maze test, combined with ultrastructural observation, hematoxylin and eosin and Nissl staining showed that WNP-10 significantly improved the learning and memory capability of the scopolamine-injured mice. The four-dimensional label-free quantification proteomics analysis identified 88 differentially expressed proteins in the WNP-10-treated group compared with scopolamine-induced impairment group. Pathway enrichment analysis and western blotting demonstrated that the WNP-10 can regulate the phosphatidylinositol-3-phosphate 5-kinase, cathepsin L, N-acetylgalactosamine 6-sulfate sulfatase and AP-3 complex subunit mu-1 expression to affect inositol phosphate metabolism, thereby maintaining lysosome ho-meostasis in scopolamine-injured mice. Notably, the results of phosphoproteomics demonstrated that WNP-10 administration resulted in the increased phosphorylation of phosphatidylinositol-3-phosphate 5-kinase. These findings provide novel insights into the underlying mechanism of memory improvement of walnut peptides.