Photocrosslinkable Kappa-Carrageenan Hydrogels for Tissue Engineering Applications
Photocrosslinkable Kappa-Carrageenan Hydrogels for Tissue Engineering Applications
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DOI：
10.1002/adhm.201200317
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发表时间：
2013-06-01
影响因子：
10
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
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作者：
Mihaila, Silvia M.;Gaharwar, Akhilesh K.;Khademhosseini, Ali
Kappa carrageenan （-CA）是一种天然聚合物，它与糖胺聚糖结构非常相似，是天然组织细胞外基质的重要成分之一。以前，已经证明-CA可以通过离子相互作用交联，形成强而脆的水凝胶。在本研究中，我们在-CA主链上引入可光交联的甲基丙烯酸酯部分，以创建物理和化学交联的水凝胶，突出其在组织工程中的应用。通过改变甲基丙烯酸基化的程度，从水化程度、溶解谱、形态、力学和流变性能等方面考察了对水凝胶交联的影响。此外，我们还研究了光交联水凝胶中培养成纤维细胞的活力。化学和物理交联程序的结合使水凝胶的形成具有高度通用的物理和化学性质，同时保持被封装细胞的活力。据我们所知，这是首次报道合成具有可控压缩模量、膨胀比和孔径分布的光交联-CA的研究。此外，通过微成型方法，可以获得空间控制的几何形状和细胞分布模式，从而使细胞材料平台的发展能够应用和定制广泛的组织工程策略。
Kappa carrageenan (-CA) is a natural-origin polymer that closely mimics the glycosaminoglycan structure, one of the most important constituents of native tissues extracellular matrix. Previously, it has been shown that -CA can crosslink via ionic interactions rendering strong, but brittle hydrogels. In this study, we introduce photocrosslinkable methacrylate moieties on the -CA backbone to create physically and chemically crosslinked hydrogels highlighting their use in the context of tissue engineering. By varying the degree of methacrylation, the effect on hydrogel crosslinking was investigated in terms of hydration degree, dissolution profiles, morphological, mechanical, and rheological properties. Furthermore, the viability of fibroblast cells cultured inside the photocrosslinked hydrogels was investigated. The combination of chemical and physical crosslinking procedures enables the formation of hydrogels with highly versatile physical and chemical properties, while maintaining the viability of encapsulated cells. To our best knowledge, this is the first study reporting the synthesis of photocrosslinkable -CA with controllable compressive moduli, swelling ratios and pore size distributions. Moreover, by micromolding approaches, spatially controlled geometries and cell distribution patterns could be obtained, thus enabling the development of cell-material platforms that can be applied and tailored to a broad range of tissue engineering strategies.