Differential matrix degradation and turnover in early cartilage lesions of human knee and ankle joints
Differential matrix degradation and turnover in early cartilage lesions of human knee and ankle joints
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DOI：
10.1002/art.20740
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发表时间：
2005-01-01
影响因子：
--
通讯作者：
Cole, AA
中科院分区：
文献类型：
--
作者：
Aurich, M;Squires, GR;Cole, AA
目标。确定踝关节（距骨）和膝关节（胫股）早期软骨病变中基质转换是否存在差异，这可能有助于解释这两个关节骨性关节炎患病率的差异。分析股骨和股骨髁病变软骨的IIB型胶原信使RNA、c端II型前胶原前肽（CPII）、胶原酶裂解新表位（Co12-3/4C（短））和变性表位（Col2-3/4m）。测定了胶原蛋白、糖胺聚糖和聚集蛋白846表位的含量。在踝关节病变中，合成标记物上调（CPII [P = 0.07]；表位846 [P小于或等于0.0001]），但在膝关节中下调（CPII [P = 0.1]；表位846 [P = 0.004]）。在膝关节病变中，胶原降解标志物上调（P = 0.008）。在磨牙基础上，与踝关节病变相比，膝关节病变的卵裂表位比变性表位多24倍。在踝关节早期软骨病变中，基质转换的上调似乎代表了修复受损基质的尝试。在踝关节病变中所见的胶原合成和聚集蛋白转换的增加在膝关节病变中是不存在的。相反，II型胶原蛋白的分裂会增加。再加上胶原变性的差异，这些变化表明在踝关节早期病变发展和膝关节退化过程中，基质组装受到重视，从而导致这些病变中基质转换的根本差异。
Objective. To determine whether there are differences in matrix turnover within early cartilage lesions of the ankle (talocrural) joint compared with the knee (tibiofemoral) joint that may help explain differences in the prevalence of osteoarthritis in these 2 joints.Methods. Cartilage removed from lesions of the tali and femoral condyles was analyzed for type IIB collagen messenger RNA, C-terminal type II procollagen propeptide (CPII), the collagenase cleavage neoepitope (Co12-3/4C(short)), and the denaturation epitope (Col2-3/4m). The content of collagen, glycosaminoglycan, and epitope 846 of aggrecan was quantitated.Results. In ankle lesions, there was an upregulation of markers of synthesis (CPII [P = 0.07]; epitope 846 [P less than or equal to 0.0001]), but these were downregulated in the knee (CPII [P = 0.1]; epitope 846 [P = 0.004]). In lesions of the knee, but not the ankle, there was an up-regulation of collagen degradation markers (P = 0.008). On a molar basis, there was 24 times more cleavage epitope than denaturation epitope in knee lesions compared with ankle lesions.Conclusion. The up-regulation of matrix turnover that is seen in early cartilage lesions of the ankle would appear to represent an attempt to repair the damaged matrix. The increase in collagen synthesis and aggrecan turnover seen in ankle lesions is absent from knee lesions. Instead, there is an increase in type II collagen cleavage. Together with the differences in collagen denaturation, these changes point to an emphasis on matrix assembly during early lesion development in the ankle and to degradation in the knee, resulting in fundamental differences in matrix turnover in these lesions.