An Insect Virus for Genetic Engineering: Developing Baculovirus Polyhedrin Substitution Vectors
An Insect Virus for Genetic Engineering: Developing Baculovirus Polyhedrin Substitution Vectors
复制标题
用于基因工程的昆虫病毒：开发杆状病毒多角体替代载体
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
M. Adang
中科院分区：
文献类型：
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作者：
L. Miller;David W. Miller;M. Adang
Baculoviruses are exceptionally attractive candidates as vectors for propagating and expressing exogenous DNAs in a eukaryotic (invertebrate) environment (Miller, 1981a). Among the features which make baculoviruses highly advantageous as recombinant DNA vector systems are (1) a covalently-closed, circular, nuclear-replicating DNA genome, (2) an extendable rod-shaped capsid, (3) a group of genes, involved in occlusion, that are nonessential for infectious virus production and thus deletable, and (4) a strong promoter which is turned on after infectious virus production and controls the synthesis of the major occlusion body protein (poly-hedrin), constituting approximately ten percent of the protein of infected cells. The replacement of the polyhedrin gene with passenger DNA was previously suggested as an approach to using baculoviruses as recombinant DNA vectors (Miller, 1981a). The initial experimental advances our laboratory has made in developing the baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) as a vector in insect cells are described herein.
