FUNCTIONAL-ROLE OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 VPU
FUNCTIONAL-ROLE OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 VPU
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DOI：
10.1073/pnas.86.13.5163
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发表时间：
1989-07-01
影响因子：
11.1
通讯作者：
HASELTINE, WA
中科院分区：
文献类型：
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作者：
TERWILLIGER, EF;COHEN, EA;HASELTINE, WA
为了研究vpu在人类免疫缺陷病毒1型（HIV-1）的复制和细胞病变中的作用，构建了感染性前病毒，这些前病毒除了能够产生vpu的蛋白质产物之外都是等基因的。显示vpu编码的蛋白质降低感染的CD 4+人T细胞中合胞体形成和细胞杀伤的速率，大大增加病毒颗粒从感染细胞的输出，并降低细胞相关病毒蛋白的积累速率。vpu蛋白反式补充了vpu- HIV-1前病毒的缺陷，但不影响缺乏vpu的猿猴免疫缺陷病毒。这些观察结果表明，vpu可能有助于艾滋病的流行，通过增加病毒的传播效率。
To investigate the role of vpu in the replication and cytopathicity of human immunodeficiency virus type 1 (HIV-1), infectious proviruses were constructed that were isogenic except for the ability to produce the protein product of vpu. The vpu-encoded proteins is shown to decrease the rate of syncytium formation and cell killing in infected CD4+ human T cells, to increase greatly the export of virus particles from infected cells, and to reduce the rate of accumulation of cell-associated viral proteins. The vpu protein complements in trans the defect in a vpu- HIV-1 provirus but does not affect the simian immunodeficiency virus, which lacks vpu. These observations suggest that vpu may contribute to the AIDS epidemic by increasing the transmission efficiency of the virus.