Terahertz Time-Domain Spectroscopy to Identify and Evaluate Anomer in Lactose
Terahertz Time-Domain Spectroscopy to Identify and Evaluate Anomer in Lactose
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DOI：
10.4236/ajac.2013.412092
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发表时间：
2013-12
期刊：
影响因子：
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通讯作者：
S. Yamauchi;Sakura Hatakeyam;Y. Imai;M. Tonouchi
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文献类型：
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作者：
S. Yamauchi;Sakura Hatakeyam;Y. Imai;M. Tonouchi
采用透射式太赫兹时域谱对一水合α-D-乳糖和无水β-D-乳糖组成的乳糖粉末进行了无损定量评价。无水β-D-乳糖的吸收峰为39.7 cm-1(1.19太赫兹)，而α-D-乳糖的吸收峰为17.1 cm-1(0.53太赫兹)和45.6厘米-1(1.37太赫兹)。经洛伦兹去卷积后，α-和β-乳糖粉的39.7 cm-1和45.6 cm-1吸收峰的积分强度唯一地依赖于乳糖粉的重量组成比。因此，用积分强度比可以准确地评价乳糖粉中α-和β-乳糖的净摩尔比。此外，对从乳糖溶液中提取的乳糖粉中的异构体含量进行了评价，并用该评价方法显示了精制和未精制的特征。
Lactose powder consisting of α-D-lactose monohydrate and anhydrous β-D-lactose was nondestructively and quantitatively evaluated by transmission-type Terahertz time-domain spectroscopy (THz-TDS). An absorption with peak at 39.7 cm-1 (1.19 THz) was assigned to be derived from anhydrous β-D-lactose, in addition to the absorptions due to α-D-lactose monohydrate with peak at 17.1 cm-1 (0.53 THz) and 45.6 cm-1 (1.37 THz). After deconvolution of the spectra using Lorentzian, integrated intensities of the absorptions with peak at 39.7 cm-1 and 45.6 cm-1 were uniquely dependent on the weight composition ratio of the α-and β-lactose powder. As a result, the net molar-ratio of the α-and β-lactose in lactose powder could be precisely evaluated by the integrated intensity ratio. Further, anomer content in lactose powder extracted from lactose solution was evaluated and the refined and unrefined features were shown by the evaluation method.