Effects of pulsed electromagnetic field (PEMF) stimulation on bone tissue like formation are dependent on the maturation stages of the osteoblasts
Effects of pulsed electromagnetic field (PEMF) stimulation on bone tissue like formation are dependent on the maturation stages of the osteoblasts
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DOI：
10.1002/bem.10032
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发表时间：
2002-07-01
影响因子：
1.9
通讯作者：
Ito, G
中科院分区：
文献类型：
--
作者：
Diniz, P;Shomura, K;Ito, G
研究了脉冲电磁场（PEMF， 15 Hz脉冲爆发，7 mT峰值）刺激对不同成熟阶段成骨细胞（MC3T3-E1细胞系）骨组织样形成的影响，以确定脉冲电磁场对骨组织样形成的刺激作用是否与细胞数量的增加和/或细胞分化的增强有关。细胞增殖（DNA含量）、分化（碱性磷酸酶活性）。测定不同时间点骨组织样形成（矿化基质面积）。PEMF处理处于增殖活跃期的成骨细胞可加速细胞增殖。细胞分化增强。增加了骨组织样的形成。分化阶段的成骨细胞经PEMF处理后，细胞分化增强，骨组织样形成增加。矿化阶段的成骨细胞经PEMF处理后，骨组织样形成减少。综上所述，PEMF在培养早期对成骨细胞有刺激作用，促进了骨组织样形成。这种刺激作用很可能与细胞分化的增强有关，而与细胞数量的增加无关。(C) 2002 Wiley-Liss。公司。
The effects of pulsed electromagnetic field (PEMF, 15 Hz pulse burst, 7 mT peak) stimulation on bone tissue-like formation on osteoblasts (MC3T3-E1 cell line) in different stages of maturation,A ere assessed to deter-mine whether the PEMF stimulatory effect on bone tissue-like formation was associated with the increase in the number of cells and/or with the enhancement of the cellular differentiation. The cellular proliferation (DNA content), differentiation (alkaline phosphatase activity). and bone tissue-like formation (area of mineralized matrix) were determined at different time points. PEMF treatment of osteoblasts in the active proliferation stage accelerated cellular proliferation. enhanced cellular differentiation. and increased bone tissue-like formation. PEMF treatment of osteoblasts in the differentiation stage enhanced cellular differentiation and increased bone tissue-like formation. PEMF treatment of osteoblasts in the mineralization stage decreased bone tissue-like formation. In conclusion, PEMF had a stimulatory effect on the osteoblasts in the early stages of culture, which increased bone tissue-like formation. This stimulatory effect was most likely associated with enhancement of the cellular differentiation, but not with the increase in the number of cells. (C) 2002 Wiley-Liss. Inc.