Streptozotocin toxicity to cultured pancreatic islets of the Syrian hamster.
Streptozotocin toxicity to cultured pancreatic islets of the Syrian hamster.
复制标题
链脲佐菌素对叙利亚仓鼠培养胰岛的毒性。
DOI：
10.1007/bf00058742
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发表时间：
1988
影响因子：
6.1
通讯作者：
Archer,MC
中科院分区：
文献类型：
--
作者：
Zucker,PF;Archer,MC
叙利亚金仓鼠的胰岛在培养中维持了很长一段时间。通过测量胰岛素分泌来评估链脲佐菌素在这些培养物中的毒性。分离后立即将胰岛暴露于1或2 mM链脲佐菌素导致胰岛素分泌的永久性和剂量相关抑制。相衬显微镜观察到，这伴有胰岛破坏。胰岛在接触链脲佐菌素前培养24小时，对毒性有保护作用。例如，将新鲜分离的胰岛暴露于2毫米链脲佐菌素导致β细胞完全破坏，而同样暴露于24小时培养期后的胰岛继续分泌胰岛素数月。然而，在暴露于0.1-0.5 mM链脲佐菌素之前，在培养中维持一周的胰岛比新鲜分离的胰岛更敏感。每周重复将培养的胰岛暴露在“无毒”浓度（0.1 mM）下，11周后胰岛素分泌持续受到抑制。
Pancreatic islets of the Syrian golden hamster were maintained in culture for extended periods of time. Toxicity of streptozotocin in these cultures was evaluated by measurement of insulin secretion. Exposure of islets to 1 or 2 mM streptozotocin immediately following isolation resulted in a permanent and dose-related inhibition of insulin secretion. This was accompanied by islet disruption as observed by phase-contrast microscopy. Culture of islets for 24 hours before streptozotocin exposure afforded protection from toxicity. For example, exposure of freshly isolated islets to 2 mM streptozotocin resulted in complete destruction of beta cells, whereas islets similarly exposed after a 24 hr culture period continued to secrete insulin for many months. Islets maintained in culture for one week before exposure to 0.1–0.5 mM streptozotocin, however, became more sensitive than freshly isolated islets. Repeated weekly exposure of cultured islets to a “non-toxic” concentration (0.1 mM) resulted in sustained suppression of insulin secretion after 11 weeks.