Expression of FAK-related non-kinase (FRNK) coincides with morphological change in the early stage of cell adhesion
Expression of FAK-related non-kinase (FRNK) coincides with morphological change in the early stage of cell adhesion
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DOI：
10.1007/s00795-006-0325-8
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发表时间：
2006-09-01
影响因子：
1.8
通讯作者：
Tachikawa, Tetsuhiko
中科院分区：
文献类型：
--
作者：
Nagoshi, Yuuki;Yamamoto, Gou;Tachikawa, Tetsuhiko
粘着斑激酶（FAK）是一种蛋白酪氨酸激酶，最近被认为通过整合素在信号转导中发挥作用。事实上，FAK通过整合素进行信号转导而参与细胞增殖和细胞运动。FAK相关非激酶（FRNK）是FAK的抑制剂。由于FRNK在细胞中的表达水平非常低，迄今为止，FRNK的研究优先通过基因过表达进行，并且组成型FRNK在细胞中的作用仍然不清楚。我们假设FRNK参与细胞与细胞外基质（ECM）的粘附，并在钟形接种后不久通过时间动力学分析研究FRNK的表达。我们发现，FRNK的表达显着增加的细胞在早期阶段的细胞粘附到ECM。这些数据表明，FRNK在细胞培养的非常早期阶段的细胞粘附中起重要作用。
Focal adhesion kinase (FAK), a protein tyrosine kinase, has recently been suggested to play a role in signal transduction through integrins. In fact, FAK is involved in cell proliferation and cell motility by performing signal transduction through integrins. FAK-related non-kinase (FRNK) has been found to be an inhibitor of FAK. As the expression level of FRNK in the cell is very low, the study of FRNK has been preferentially performed by gene overexpression, up to the present, and the role of constitutive FRNK in cells remains unclear. We hypothesized that FRNK is involved in the adhesion of cells to the extracellular matrix (ECM) and investigated the expression of FRNK by time kinetic analysis shortly after bell seeding. We found that FRNK expression was significantly increased in the cells during the early stage of cell adhesion to the ECM. These data indicated that FRNK plays an important role in cell adhesion during the very early stages of cell culture.