Surface modification of gold nanorods using layer-by-layer technique for cellular uptake
Surface modification of gold nanorods using layer-by-layer technique for cellular uptake
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DOI：
10.1007/s11051-007-9227-5
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发表时间：
2008-02
影响因子：
2.5
通讯作者：
Hironobu Takahashi;T. Niidome;T. Kawano;S. Yamada;Y. Niidome
中科院分区：
文献类型：
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作者：
Hironobu Takahashi;T. Niidome;T. Kawano;S. Yamada;Y. Niidome
金纳米棒 (NR) 是棒状金纳米粒子，采用逐层技术用牛血清白蛋白 (BSA) 和聚乙烯亚胺 (PEI) 进行修饰。从吸收光谱和zeta电位测量可以明显看出，NRs被这些聚合物包裹，而NRs没有聚集。通过吸收光谱验证，经过 BSA 修饰后，表面修饰的 NR（BSA-NR）在生化条件下分散良好，没有聚集。用 PEI 进一步修饰可提供带正电荷的 NR (PEI-NR)。 PEI-NRs 的透射电子显微镜图像显示，表面改性不会影响初始 NRs 形状的变化。此外，PEI-NR 在生化条件下保留了 BSA-NR 的胶体稳定性。我们评估了 PEI-NRs 与 HeLa 细胞的转染活性。从基因表达实验的结果来看，很明显，BSA对NRs的稳定作用以及PEI的进一步修饰实现了NRs转染到培养细胞中。此外，细胞对 NR 的摄取使得能够利用 NR 的光散射进行细胞成像。
Gold nanorods (NRs), rod-shaped gold nanoparticles, were modified with bovine serum albumin (BSA) and polyethylenimine (PEI) using layer-by-layer technique. From absorption spectroscopy and zeta potential measurements, it was obvious that NRs were wrapped with these polymers without aggregation of NRs. Following BSA modification, the surface-modified NRs (BSA-NRs) were well-dispersed without aggregation in biochemical conditions, verified from absorption spectroscopy. Further modification with PEI provided positively charged NRs (PEI-NRs). A transmission electron microscopy image of PEI-NRs revealed that the surface modification did not affect changing the shape of the initial NRs. In addition, the PEI-NRs retained the colloidal stability of BSA-NRs in biochemical conditions. We have evaluated that transfection activity of PEI-NRs with HeLa cells. From results of gene expression experiments, it was obvious that the stabilization of NRs by BSA and further modification with PEI realized transfection using NRs into cultured cells. Moreover, the cellular uptake of NRs enabled cellular imaging using light scattering from the NRs.