Proteolytic processing of presenilin-1 (PS-1) is not associated with Alzheimer's disease with or without PS-1 mutations
Proteolytic processing of presenilin-1 (PS-1) is not associated with Alzheimer's disease with or without PS-1 mutations
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DOI：
10.1016/s0014-5793(97)01378-1
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发表时间：
1997-11-24
期刊：
影响因子：
3.5
通讯作者：
Mori, H
中科院分区：
文献类型：
--
作者：
Okochi, M;Ishii, K;Mori, H
脑早老素-1 蛋白 (PS-1) 通常由 M-r 28 kDa 的氨基末端片段 (NTF) 和 18 kDa 的羧基末端片段 (CTF) 组成。我们分析了早发性家族性阿尔茨海默病（FAD）有和没有PS-1突变的大脑中的人类PS-1，以研究突变的PS-1是否代谢异常，发现大脑PS-1被裂解成NTF和CTF两个片段，与人脑中PS-1突变的发生无关，最近发现一小部分PS-1 nas受到与凋亡相关的半胱氨酸caspase-3的另一种处理蛋白酶。最近发现早老素 2 (PS-2) 上的伏尔加-德国突变会影响 caspase-3 PS-2 片段的增加，与此相反，PS-1 突变并未引起 PS-1 片段的显着变化。我们得出结论，细胞凋亡后 PS-1 断裂和其他（可能是 caspase-3 介导的）消化的发生与 PS-1 突变无关。 (C) 1997 年欧洲生化学会联合会。
Cerebral presenilin-1 protein (PS-1) is normally composed of the amino-terminal fragment (NTF) with M-r 28 kDa and the carboxy-terminal fragment (CTF) with 18 kDa. We analyzed human PS-1 in brains with early-onset familial Alzheimer's disease (FAD) with and without PS-1 mutations to study whether mutated PS-1 was abnormally metabolized, Cerebral PS-1 were found to be cleaved into two fragments of NTF and CTF independently of the occurrence of PS-1 mutation in human brains, A small portion of PS-1 nas recently found to suffer another processing by caspase-3, an apoptosis-related cysteine protease. In contrast to the recent finding that the Volga-German mutation on presenilin-2 (PS-2) affects the increasing caspase-3 PS-2 fragment, the PS-1 mutation did not cause a significant change in PS-1 fragmentation. We conclude that PS-1 fragmentation and other (probably caspase-3-mediated) digestion following apoptosis occur independently of PS-1 mutations. (C) 1997 Federation of European Biochemical Societies.