GsSnRK1 interplays with transcription factor GsERF7 from wild soybean to regulate soybean stress resistance
GsSnRK1 interplays with transcription factor GsERF7 from wild soybean to regulate soybean stress resistance
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GsSnRK1与野生大豆转录因子GsERF7相互作用调节大豆抗逆性
DOI：
10.1111/pce.13726
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发表时间：
2020-02-20
影响因子：
7.3
通讯作者：
Ding, Xiaodong
中科院分区：
文献类型：
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作者：
Feng, Xu;Feng, Peng;Ding, Xiaodong
尽管SnRK1的功能和调控已经在多种植物中得到了研究，但其响应非生物胁迫的分子机制尚不清楚。在这项工作中，我们从野生大豆cDNA文库中鉴定了一个AP2/ERF结构域蛋白（命名为GsERF7）与GsSnRK1相互作用。GsERF7基因在野生大豆根中主要表达，对乙烯、盐和碱有反应。GsERF7结合GCC顺式作用元件，可被GsSnRK1在S36上磷酸化。GsERF7的磷酸化促进了其从细胞质向细胞核的易位，增强了其转激活活性。当GsSnRK1（wt）和GsERF7（wt）在大豆幼苗毛状根中共表达时，GsSnRK1（wt）和GsERF7（wt）促进植物产生比其突变物种更高的盐和碱胁迫耐受性，这表明GsSnRK1可能作为GsERF7的生化和遗传上游激酶调节植物对环境胁迫的适应。此外，还测定了胁迫处理后转基因大豆毛状根中具有代表性的非生物胁迫响应基因和激素合成基因的表达模式变化。这些结果将有助于我们了解SnRK1激酶如何通过激活下游因子的生物学功能在调控植物抗逆性中发挥重要作用的分子机制。
Although the function and regulation of SnRK1 have been studied in various plants, its molecular mechanisms in response to abiotic stresses are still elusive. In this work, we identified an AP2/ERF domain-containing protein (designated GsERF7) interacting with GsSnRK1 from a wild soybean cDNA library. GsERF7 gene expressed dominantly in wild soybean roots and was responsive to ethylene, salt, and alkaline. GsERF7 bound GCC cis-acting element and could be phosphorylated on S36 by GsSnRK1. GsERF7 phosphorylation facilitated its translocation from cytoplasm to nucleus and enhanced its transactivation activity. When coexpressed in the hairy roots of soybean seedlings, GsSnRK1(wt) and GsERF7(wt) promoted plants to generate higher tolerance to salt and alkaline stresses than their mutated species, suggesting that GsSnRK1 may function as a biochemical and genetic upstream kinase of GsERF7 to regulate plant adaptation to environmental stresses. Furthermore, the altered expression patterns of representative abiotic stress-responsive and hormone-synthetic genes were determined in transgenic soybean hairy roots after stress treatments. These results will aid our understanding of molecular mechanism of how SnRK1 kinase plays a cardinal role in regulating plant stress resistances through activating the biological functions of downstream factors.