n-3 PUFA and caloric restriction diet alters lipidomic profiles in obese men with metabolic syndrome: a preliminary open study
n-3 PUFA and caloric restriction diet alters lipidomic profiles in obese men with metabolic syndrome: a preliminary open study
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DOI：
10.1007/s00394-019-02149-4
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发表时间：
2020-10-01
影响因子：
5
通讯作者：
Huang, S. -Y.
中科院分区：
文献类型：
--
作者：
Shabrina, A.;Tung, T. -H.;Huang, S. -Y.
目的对于代谢综合征(METS)患者，改变他们饮食中的常量营养素组成可能会改善代谢异常。临床评估的常用方法只测量总脂浓度，而忽略了对总浓度有贡献的个别物种。因此，为了预测限制卡路里(CR)和摄入鱼油后蛋氨酸的改善，我们使用脂类组学研究了血浆脂类的变化，并确定了潜在的脂代谢产物。方法采用超高效液相色谱-串联质谱仪对12周以上临床试验的血浆样品进行脂质组学分析。受试者被随机分为两组：CR组(n=12)和CR加鱼油组(CRF，n=9)。测量人体测量学和临床参数，并与血浆脂质组学数据进行相关分析。结果与治疗前比较，两组患者的体重、腰围、血压、白介素6水平均有显著差异(P<0.05)，而甘油三酯(TG)水平仅CRF组显著降低(P均<0.05)。共鉴定出138种脂类。摄入鱼油后，含有长链多不饱和脂肪酸的物种水平显著升高--超过两倍，包括甘油三酯(60：9)和磷脂酰胆碱(p40：6)(allq<；0.05)。摄入鱼油后，TG(60：9)与体重、体重指数、血压、糖化血红蛋白呈负相关。结论CR和鱼油可改善METS患者的人体测量参数、血压和血脂水平。特定的脂质种类，如含甘油三酯的二十二碳五烯酸的水平在干预后升高，并与蛋氨酸的特征呈负相关。TG(60：9)可作为一种脂代谢产物来预测摄入CR和鱼油后蛋氨酸水平的改善。
Purpose For people with metabolic syndrome (MetS), altering the macronutrient composition of their diets might ameliorate metabolic abnormalities. The common method of clinical assessment only measures total lipid concentrations but ignores the individual species that contribute to these total concentrations. Thus, to predict the amelioration of MetS following caloric restriction (CR) and the intake of fish oil, we used lipidomics to investigate changes in plasma lipids and identify potential lipid metabolites. Methods Lipidomics was performed using ultra-high-performance liquid chromatography-tandem mass spectrometry on plasma samples from a clinical trial conducted over 12 weeks. Subjects were randomized into two groups: CR (n = 12) and CR with fish oil (CRF,n = 9). Anthropometric and clinical parameters were measured and correlated with plasma lipidomics data. Results Compared with baseline, significant differences were observed in body weight, waist circumference, blood pressure and interleukin-6 in both groups, but triglyceride (TG) levels significantly decreased in only the CRF group (allp < 0.05). A total of 138 lipid species were identified. Levels of species containing long-chain polyunsaturated fatty acids were significantly elevated-greater than twofold-following fish oil intake, these included TG (60:9) and phosphatidylcholine (p40:6) (allq < 0.05). TG (60:9) tended to correlate negatively with body weight, body mass index, blood pressure, and HbA1c following fish oil intake. Conclusion CR and fish oil can ameliorate MetS features, including anthropometric parameters, blood pressure, and blood lipid concentrations. The levels of particular lipid species such as TG-containing docosapentaenoic acid were elevated post-intervention and negatively associated with MetS features. TG (60:9) may be proposed as a lipid metabolite to predict amelioration in MetS following the intake of CR and fish oil.