Hrs1p/Mcp6p on the Meiotic SPB Organizes Astral Microtubule Arrays for Oscillatory Nuclear Movement
Hrs1p/Mcp6p on the Meiotic SPB Organizes Astral Microtubule Arrays for Oscillatory Nuclear Movement
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DOI：
10.1016/j.cub.2005.07.058
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发表时间：
2005-08
期刊：
影响因子：
9.2
通讯作者：
Kayoko Tanaka;Toshiki Kohda;A. Yamashita;N. Nonaka;Masayuki Yamamoto
中科院分区：
文献类型：
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作者：
Kayoko Tanaka;Toshiki Kohda;A. Yamashita;N. Nonaka;Masayuki Yamamoto
微管和运动蛋白Dynein在细胞核和细胞质细胞器的运动和定位中起着关键作用。在分裂酵母中，在减数分裂前期观察到称为马尾核运动(HNM)的核的振荡运动[1，2]。HNM由来自纺锤体极体(SPB)的星形微管阵列领导，SPB是酵母中相当于中心体的细胞器，由动力蛋白-动力蛋白复合体辅助，被认为是促进有效减数分裂重组所必需的同源染色体的比对[3-8]。在这里，我们证明了减数分裂特异的SPB组分Hrs1p(也称为Mcp6p[9])是将微管重塑为马尾-星形阵列(HAA)的关键分子。Hrs1p缺失会损害HAA的形成，导致HNM受损。Hrs1p在有丝分裂细胞周期中的异位表达导致HAA样星形微管阵列的形成，从而驱动间期细胞的振荡核运动。Hrs1p与γ-微管蛋白环复合体的组分(γ-TURC)相互作用，也与减数分裂的Spb组分相互作用。我们认为，Hrs1p通过稳定SPB和微管负端之间的连接，促进了HAA的形成，从而导致了旺盛的HNM。
Microtubules and the motor protein dynein play pivotal roles in the movement and positioning of the nucleus and cytoplasmic organelles in a cell. In fission yeast, oscillatory movement of the nucleus termed horsetail nuclear movement (HNM) has been observed during meiotic prophase [1, 2]. HNM is led by an astral microtubule array emanating from the spindle pole body (SPB), a centrosome-equivalent organelle in yeasts, aided by the dynein-dynactin complex, and is proposed to facilitate the alignment of homologous chromosomes necessary for efficient meiotic recombination [3–8]. Here we show that a meiosis-specific SPB component Hrs1p (also known as Mcp6p [9]) is a key molecule to remodel microtubules into the horsetail-astral array (HAA). Deletion of Hrs1p impaired HAA formation, leading to compromised HNM. Ectopic expression of Hrs1p during the mitotic cell cycle resulted in the formation of a HAA-like astral microtubule array, which drove an oscillatory nuclear movement in interphase cells. Hrs1p interacted with components of the γ-tubulin ring complex (γ-TuRC) as well as with a meiotic SPB component. We propose that Hrs1p facilitates formation of the HAA, responsible for the vigorous HNM, by stabilizing connection between the SPB and minus ends of microtubules.