IDENTIFICATION AND QUANTIFICATION OF ACTIN ISOFORMS IN VERTEBRATE CELLS AND TISSUES
IDENTIFICATION AND QUANTIFICATION OF ACTIN ISOFORMS IN VERTEBRATE CELLS AND TISSUES
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DOI：
10.1002/jcb.240340205
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发表时间：
1987-06-01
影响因子：
4
通讯作者：
BULINSKI, JC
中科院分区：
文献类型：
--
作者：
OTEY, CA;KALNOSKI, MH;BULINSKI, JC
细胞骨架蛋白肌动蛋白在脊椎动物中以六种不同的亚型存在，由于整体序列同源性很高（> 90%），因此很难最终确定。我们使用 IEF 免疫印迹结合一组异构体特异性和选择性抗体来分析成年大鼠九种组织的肌动蛋白异构体组成。在三种非肌肉组织（肺、脾和睾丸）中，我们检测到了一种以前未报告的亚型，我们将其鉴定为平滑肌α。IEF 免疫印迹技术也用于量化这九种大鼠组织中表达的亚型的比例。
The cytoskeletal protein actin exists in vertebrates as six different isoforms, which are difficult to identify conclusively because of a high degree (> 90%) of overall sequence homology. We have used IEF immunoblotting in combination with a panel of isoform-specific and -selective antibodies to analyze the actin isoform composition of nine tissues from adult rat. In three nonmuscle tissues (lung, spleen, and testis), we detected a previously unreported isoform that we identified as smooth muscle .alpha.. The IEF immunoblot technique was also used to quantify the proportions of the isoforms expressed in these nine rat tissues.