Detection of secretory immunoglobulin A in human colostrum as mucosal immune response against proteins of the type III secretion system of Salmonella, Shigella and enteropathogenic Escherichia coli.
Detection of secretory immunoglobulin A in human colostrum as mucosal immune response against proteins of the type III secretion system of Salmonella, Shigella and enteropathogenic Escherichia coli.
复制标题
人类初乳中分泌免疫球蛋白A的检测是针对沙门氏菌，志贺氏菌和肠病毒性大肠杆菌III型分泌系统的蛋白质的粘膜免疫反应。
DOI：
10.1097/inf.0b013e318293306c
复制
发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Cleary TG
中科院分区：
文献类型：
--
作者：
Durand D;Ochoa TJ;Bellomo SM;Contreras CA;Bustamante VH;Ruiz J;Cleary TG
一些肠道病原体使用三型分泌系统(T3SS)来分泌蛋白质，使它们能够与肠细胞相互作用，促进细菌附着或细胞内生存。这些蛋白是志贺氏菌的沙门氏菌侵袭蛋白(Sip)、侵袭质粒抗原(IPA)和致病性大肠埃希菌(EPEC)的大肠杆菌分泌蛋白(ESP)。以前没有研究定义初乳SIgA对这三种主要肠道病原体的存在。评价初乳中SIgA对沙门氏菌、志贺氏菌和EPEC T3SS蛋白的影响。我们收集了76份来自秘鲁利马产后妇女的初乳样本。这些样品与从细菌培养上清液中提取的T3SS蛋白反应，并用Western Blot进行鉴定。抗SipA 75例(99%)，SIPC 62例(82%)，SipB 31例(41%)，志贺氏菌IPAC 70例(92%)，IPAB 68例(89%)，IPAA 66例(87%)，iPad 41例(54%)，EPEC ESPC 70例(92%)，EspB-D 65例(86%)，EspA 41例(54%)。10%的样本对所有蛋白质进行了抗体评估；42%的样本对除一种蛋白质外的所有蛋白质进行了抗体评估。所有这些蛋白质的检测结果均为阴性。在这项研究中，在产后妇女的初乳中发现了针对这些多种肠道病原体的异常高频率的抗体，这表明了免疫记忆和先前接触过这些病原体，以及它可能对感染的保护作用。
Some enteropathogens use the type three secretion system (T3SS) to secrete proteins that allows them to interact with enterocytes and promote bacterial attachment or intracellular survival. These proteins are Salmonella invasion proteins (Sip), invasion plasmid antigens (Ipa) of Shigella and E. coli secreted proteins (Esp) of enteropathogenic E. coli (EPEC). There are no previous studies defining the presence of colostral sIgA against all these three major enteric pathogens. To evaluate the presence of sIgA in colostrum against proteins of the T3SS of Salmonella, Shigella and EPEC. We collected 76 colostrum samples from puerperal women in Lima, Peru. These samples were reacted with T3SS proteins extracted from bacterial culture supernatants and evaluated by Western Blot. Antibodies were detected against Salmonella antigens SipA in 75 samples (99%), SipC in 62 (82%) and SipB in 31 (41%); against Shigella antigens IpaC in 70 (92%), IpaB in 68 (89%), IpaA in 66 (87%) and IpaD in 41 (54%); and against EPEC EspC in 70 (92%), EspB-D in 65 (86%) and EspA in 41 (54%). 10% of samples had antibodies against all proteins evaluated; and 42% against all except one protein. There was no sample negative to all these proteins. The extraordinarily high frequency of antibodies in colostrum of puerperal women detected in this study against these multiple enteric pathogens, shows evidence of immunological memory and prior exposure to these pathogens, in addition to its possible protective role against infection.