Immunohistologicalstudy of small Rho GTPases and beta-catenin during regeneration of the rat submandibular gland
Immunohistologicalstudy of small Rho GTPases and beta-catenin during regeneration of the rat submandibular gland
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小Rho GTPases和β-catenin在大鼠颌下腺再生过程中的免疫组织学研究
DOI：
10.1007/s10735-012-9437-8
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发表时间：
2012
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影响因子：
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et al.
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作者：
Hiroshi Shiratsuchi;Osamu Shimizu;et al.
我们的研究用免疫组织化学方法研究了小Rho GTP酶和β-连环蛋白在大鼠颌下腺再生过程中的定位模式。术后7d取材，分别于术后0d、3d、7d、11d、14d取材，进行再生研究。新生腺泡细胞第3~7天RhoA表达较强，RhoC表达较弱，11~14天RhoA和RhoC在基底部和胞浆均呈中等强度表达，RhoB在新形成和成熟腺泡细胞胞浆中分别呈强阳性和中等阳性表达，在管状结构(DLSS)和夹层导管(ICD)中呈强阳性表达。在细胞间和亚细胞区均可检测到β-catenin，但在新形成的腺泡细胞中未检测到Rac1-catenin。β免疫标记逐渐减少，RAC1-连环蛋白染色变强。P-rac1是一种磷酸化的rac1，存在于新生腺泡细胞的胞浆中。在DLS和ICD的顶端和亚细胞区，检测到了Rac1和β-连环蛋白。这些结果表明，RhoA和RhoC可能参与了再生腺泡细胞基底外侧部位的肌动蛋白细胞骨架，而RhoB可能在再生腺泡细胞以及DLS和ICD中起着独特的作用。细胞-细胞区的Rac1和β-catenin可能在细胞间的黏附、再生腺泡细胞的分化以及DLS和ICD顶端和亚细胞区的肌动蛋白重建中发挥重要作用。
Our study immunohistochemically evaluated the localization patterns of small Rho GTPases and β-catenin during regeneration of the rat submandibular gland. After 7 days of obstruction, regenerating glands were collected at days 0, 3, 7, 11 and 14 after duct release to study regeneration. RhoA was detected strongly and RhoC was detected weakly in the cytoplasm of newly formed acinar cells from day 3 to 7, and both RhoA and RhoC at the basal site and cytoplasm were detected moderately from day 11 to 14. RhoB was detected strongly and moderately in the cytoplasm of newly formed and matured acinar cells, respectively, and detected strongly in duct-like structures (DLSs) and intercalated ducts (ICDs). Rac1 was detected at the cell–cell and subcellular region, but β-catenin was not observed in newly formed acinar cells. Rac1 immunolabeling gradually reduced, and the β-catenin staining pattern became stronger. p-Rac1, a phosphorylated form of Rac1, was observed in the cytoplasm of newly formed acinar cells. At apical and subcellular region of DLSs and ICDs, Rac1 and β-catenin were detected. These findings suggest that RhoA and RhoC might be involved in the actin cytoskeleton at the basolateral site of regenerating acinar cells, and RhoB might play a unique role in regenerating acinar cells and in DLSs and ICDs. Rac1 and β-catenin at the cell–cell region might play important roles in cell–cell adhesion and the differentiation of regenerating acinar cells, as well as actin reconstruction at apical and subcellular regions of DLSs and ICDs.