Spatiotemporal Analysis of Caveolae Dynamics Using Total Internal Reflection Fluorescence Microscopy
Spatiotemporal Analysis of Caveolae Dynamics Using Total Internal Reflection Fluorescence Microscopy
复制标题
使用全内反射荧光显微镜对小凹动力学进行时空分析
DOI：
10.1007/978-1-0716-0732-9_6
复制
发表时间：
2020
影响因子：
--
通讯作者：
Suetsugu S.
中科院分区：
文献类型：
--
作者：
Senju Y;Suetsugu S.
全内反射荧光显微镜能够分析在质膜处或附近发生的细胞事件的定位和动态。全内反射荧光显微镜专门照亮玻璃表面附近的分子，从而减少背景荧光，并能够以高信噪比观察玻璃附着细胞中的质膜。在这里，我们描述了应用全内反射荧光显微镜分析的动态小窝，发挥重要的生理功能，包括膜张力缓冲，内吞作用，并在质膜上的信号。
Total internal reflection fluorescence microscopy enables to analyze the localizations and dynamics of cellular events that occur at or near the plasma membrane. Total internal reflection fluorescence microscopy exclusively illuminates molecules in the close vicinity of the glass surface, thereby reducing background fluorescence and enabling observation of the plasma membrane in the glass-attached cells with a high signal-to-noise ratio. Here, we describe the application of total internal reflection fluorescence microscopy to analyze the dynamics of caveolae, which play essential physiological functions, including membrane tension buffering, endocytosis, and signaling at the plasma membrane.