Plasma membrane proteins and glycoproteins from Chinese hamster cells sensitive and resistant to actinomycin D.
Plasma membrane proteins and glycoproteins from Chinese hamster cells sensitive and resistant to actinomycin D.
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中国仓鼠细胞质膜蛋白和糖蛋白对放线菌素 D 敏感和耐药。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
J. L. Biedler
中科院分区：
文献类型：
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作者：
R. Peterson;J. L. Biedler
已从自发转化的DC-3F亲本细胞系和对放线菌素D具有高水平获得性抗性的DC-3F/AD X系的中国仓鼠细胞中分离质膜蛋白和糖蛋白。等密度离心后，两种细胞系的质膜制备物条带均为1.16 μ g/ml。我们目前的证据表明，亮氨酰肽骨干的药物敏感细胞和耐药DC-3F/AD X细胞的差异。此外，十二烷基钠凝胶电泳显示两种细胞系的质膜糖肽存在差异。耐药细胞合成的表面糖肽比药物敏感细胞上的主要糖肽大得多。这两种细胞系都缺乏5 '-核苷酸酶和碱性磷酸酶活性。质膜蛋白差异在耐药细胞中的作用进行了讨论。
Plasma membrane proteins and glycoproteins have been isolated from Chinese hamster cells of the spontaneously transformed DC-3F parental cell line and the DC-3F/AD X line with a high level of acquired resistance to actinomycin D. Plasma membrane preparations from both cell lines band at 1.16 g/ml after isopycnic centrifugation. We present evidence to indicate differences in the leucylpeptide backbones of the antibiotic-sensitive cells and the drug-resistant DC-3F/AD X cells. In addition, there are differences in the plasma membrane glycopeptides of the two cell lines as revealed by sodium dodecyl gel electrophoresis. Drug-resistant cells synthesize a surface glycopeptide which is much larger than the major one present on the drug-sensitive cells. Both of these cell lines are devoid of 5'-nucleotidase and alkaline phosphatase activities. The role of plasma membrane protein differences in drug-resistant cells is discussed.