BIOGEOGRAPHY OF THE SOUTHEASTERN UNITED STATES: A COMPARISON OF SALAMANDER PHYLOGEOGRAPHIC STUDIES
BIOGEOGRAPHY OF THE SOUTHEASTERN UNITED STATES: A COMPARISON OF SALAMANDER PHYLOGEOGRAPHIC STUDIES
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美国东南部的生物地理学：蝾螈系统地理学研究的比较
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
E. Routman
中科院分区：
文献类型：
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作者：
Michele F. Donovan;R. D. Semlitsch;E. Routman
摘要对美国东南部物种的大部分地理学研究表明，线粒体DNA变异存在简单的东西划分。然而，对斑点钝口蝾螈的研究得出了一个更复杂的模式，其中包括来自密苏里州和阿肯色州中央高地以及沿海平原的种群之间的密切关系，这些种群被遗传上不同的中心种群分开。我们通过调查近缘种A的线粒体DNA（mtDNA）变异来测试这一观察结果的普遍性。距骨对钝口吸虫特异性基因间区进行了扩增和测序。得到的26个单倍型从380到800个碱基对不等，包括外群在内的比对需要101个插入/缺失。单倍型之间的序列差异在0.001 ~ 0.758之间。种群分布广泛（θ = 0.64）。系统发育分析talpoideum mtDNA序列揭示了中部高地和沿海平原人群之间的密切关系。这一结果与A的结果相似。maculatum，而A. talpoideum分支与其姐妹分支没有很好的区别。我们讨论了两个Ambystoma物种和以前的研究之间的差异和相似之处，并呼吁增加对多个物种的生态相似的一种方式来检测微妙的地理事件的关注。通讯编辑：B。博文
Abstract Most phylogeographic studies of species from the southeastern United States have shown a simple east-west division of mtDNA variation. However, a study of the salamander Ambystoma maculatum resulted in a more complex pattern that includes a close affinity between populations from the Central Highlands of Missouri and Arkansas and the Coastal Plain separated by a genetically distinct central group of populations. We test the generality of this observation by surveying mitochondrial DNA (mtDNA) variation in the closely related species A. talpoideum. An Ambystoma-specific intergenic spacer was amplified and sequenced. The 26 resulting haplotypes varied from 380 to 800 base pairs, and alignments, including the outgroup, required 101 insertions/deletions. Sequence divergence among haplotypes ranged from 0.001 to 0.758. Population subdivision was extensive (θ = 0.64). Phylogenetic analysis of A. talpoideum mtDNA sequence reveals a close relationship between the populations from the Central Highlands and the Coastal Plain. This result is similar to that obtained for A. maculatum, although the A. talpoideum clade is not as well differentiated from its sister clades. We discuss the differences and similarities between the two Ambystoma species and previous studies and call for increased focus on multiple species with similar ecologies as a way to detect subtle biogeographic events. Corresponding Editor: B. Bowen