Developmental expression of HpNanos, the Hemicentrotus pulcherrimus homologue of nanos
Developmental expression of HpNanos, the Hemicentrotus pulcherrimus homologue of nanos
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DOI：
10.1016/j.modgep.2005.10.003
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发表时间：
2006-06-01
影响因子：
1.2
通讯作者：
Yamamoto, T
中科院分区：
文献类型：
--
作者：
Fujii, T;Mitsunaga-Nakatsubo, K;Yamamoto, T
从原肠胚 cDNA 文库中分离出 Hemicentrotus pulcherrimus nanos 同源物 (HpNanos)，其编码含有两个 CCHC 锌指基序的蛋白质。报道了胚胎发育过程中 HpNanos mRNA 的积累和空间表达模式。发育性 Northern blot 分析显示，HpNanos mRNA 在囊胚阶段显着积累，然后在间充质囊胚阶段丰度下降。 HpNanos mRNA 表达的第二阶段发生在原肠胚形成期间，此后表达恢复到低水平。整体原位杂交表明，HpNanos 只在囊胚阶段的 4 至 6 个小微米后代细胞中表达。 HpNanos 的表达仅限于体腔囊，在棱柱阶段，体腔囊产生成体雏形腹侧表面的中胚层。这些结果表明，HpNanos 的表达将有助于未来分析海胆发育过程中小微米后代细胞的功能和生殖细胞的起源。 (c) 2006 Elsevier B.V. 保留所有权利。
The Hemicentrotus pulcherrimus homologue of nanos (HpNanos), that encodes a protein containing two CCHC zinc finger motifs, was isolated from a gastrula cDNA library. The accumulation of HpNanos mRNA during embryonic development and the spatial expression pattern are reported. Developmental northern blot analysis revealed that HpNanos mRNA markedly accumulated during the blastula stages, and then decreased in abundance at the mesenchyme blastula stage. The second phase of HpNanos mRNA expression occurred during gastrulation, after which the expression returned to a low level. Whole-mount in situ hybridization showed that the HpNanos was exclusively expressed in four to six small micromere-descendant cells at the blastula stage. The expression of HpNanos was restricted to the coelomic pouch, which gives rise to the mesoderm of the ventral surface of the adult rudiment, at the prism stage. These results suggest that HpNanos expression will be instrumental for future analyses of the function of small micromere-descendant cells and of the origin of germ cells during sea urchin development. (c) 2006 Elsevier B.V. All rights reserved.