Silencing NADPH-cytochrome P450 reductase results in reduced acaricide resistance in Tetranychus cinnabarinus (Boisduval).
Silencing NADPH-cytochrome P450 reductase results in reduced acaricide resistance in Tetranychus cinnabarinus (Boisduval).
复制标题
沉默 NADPH-细胞色素 P450 还原酶可降低朱砂叶螨 (Boisduval) 的杀螨剂抗性
DOI：
10.1038/srep15581
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发表时间：
2015-10-23
影响因子：
4.6
通讯作者：
He L
中科院分区：
文献类型：
--
作者：
Shi L;Zhang J;Shen G;Xu Z;Wei P;Zhang Y;Xu Q;He L
细胞色素P450单加氧酶（P450）参与了对杀虫剂的代谢抗性，在催化氧化反应时需要NADPH细胞色素P450还原酶（CPR）转移电子。朱砂叶螨（Tetranychus cinnabarinus）是世界范围内重要的作物和蔬菜害虫螨，其对杀螨剂的抗性发展很快。然而，CPR在朱砂金蝇抗杀螨性形成中的作用尚不清楚。在本研究中，我们从朱砂树（T. cinnabarinus）中克隆并鉴定了一个全长cDNA （TcCPR）。TcCPR的表达在朱砂金蝇的所有发育阶段都可以检测到，但在卵中的表达要低得多。与敏感菌株SS相比，经甲氰菊酯处理的耐甲氰菊酯菌株TcCPR表达上调，诱导能力增强。投喂双链RNA能有效地沉默朱砂赤潮菌TcCPR的转录，导致赤潮菌株p450的活性降低，对甲氰菊酯的敏感性增加，而易感菌株则没有。本研究结果首次证明了TcCPR的下调导致耐药螨对甲氰菊酯的敏感性增加。TcCPR可作为开发新型农药的新靶点。
Cytochrome P450 monooxygenases (P450s) are involved in metabolic resistance to insecticides and require NADPH cytochrome P450 reductase (CPR) to transfer electrons when they catalyze oxidation reactions. The carmine spider mite, Tetranychus cinnabarinus is an important pest mite of crop and vegetable plants worldwide and its resistance to acaricides has quickly developed. However, the role of CPR on the formation of acaricide-resistance in T. cinnabarinus is still unclear. In this study, a full-length cDNA encoding CPR was cloned and characterized from T. cinnabarinus (designated TcCPR). TcCPR expression was detectable in all developmental stages of T. cinnabarinus, but it’s much lower in eggs. TcCPR was up-regulated and more inducible with fenpropathrin treatment in the fenpropathrin-resistant (FeR) strain compared with the susceptible SS strain. Feeding of double-strand RNA was effective in silencing the transcription of TcCPR in T. cinnabarinus, which resulted in decreasing the activity of P450s and increasing the susceptibility to fenpropathrin in the FeR strain but not in the susceptible strain. The current results provide first evidence that the down-regulation of TcCPR contributed to an increase of the susceptibility to fenpropathrin in resistant mites. TcCPR could be considered as a novel target for the development of new pesticides.