Genome sequence of Halobacterium species NRC-1
Genome sequence of Halobacterium species NRC-1
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DOI：
10.1073/pnas.190337797
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发表时间：
2000-10-24
影响因子：
11.1
通讯作者：
DasSarma, S
中科院分区：
文献类型：
--
作者：
Ng, WV;Kennedy, SP;DasSarma, S
我们报告了极端嗜盐菌Halobacterium sp. NRC-1的完整序列，该菌具有一个动态的2，571，010-bp基因组，包含代表12个家族的91个插入序列，并组织成一个大染色体和2个相关的微型染色体。盐杆菌NRC-1基因组编码2,630种预测蛋白，其中36%与任何先前报道的蛋白无关。对基因组序列的分析表明存在氨基酸的摄取和利用途径、活性钠质子反向转运体和钾摄取系统、复杂的感光和信号转导途径以及DNA复制。转录和翻译系统类似于更复杂的真核生物。整个蛋白质组的比较表明，这种嗜盐菌与革兰氏阳性枯草芽孢杆菌和其他细菌的额外的相似性明确的古细菌的性质。培养盐杆菌的容易性和在实验室中进行遗传操作的方法的可用性，包括基因敲除和替换的构建，表明这种嗜盐菌可以作为古菌中的一个极好的模型系统。
We report the complete sequence of an extreme halophile, Halobacterium sp. NRC-1, harboring a dynamic 2,571,010-bp genome containing 91 insertion sequences representing 12 families and organized into a large chromosome and 2 related minichromosomes.:The Halobacterium NRC-1 genome codes for 2,630 predicted proteins, 36% of which are unrelated to any previously reported. Analysis of the genome sequence shows the presence of pathways for uptake and utilization of amino acids, active sodium proton antiporter and potassium uptake systems, sophisticated photosensory and signal transduction pathways, and DNA replication.: transcription, and translation systems resembling more complex eukaryotic organisms. Whole proteome comparisons show the definite archaeal nature of this halophile with additional similarities to the Gram-positive Bacillus subtilis and other bacteria. The ease of culturing Halobacterium and the availability of methods for its genetic manipulation in the laboratory, including construction of gene knockouts and replacements, indicate this halophile can-serve as an excellent model system among the archaea.