Construction of a novel cationic polymeric liposomes formed from PEGylated octadecyl‐quaternized lysine modified chitosan/cholesterol for enhancing storage stability and cellular uptake efficiency
Construction of a novel cationic polymeric liposomes formed from PEGylated octadecyl‐quaternized lysine modified chitosan/cholesterol for enhancing storage stability and cellular uptake efficiency
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DOI：
10.1002/bit.22773
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发表时间：
2010-08
影响因子：
3.8
通讯作者：
Hanjie Wang;P. Zhao;Xiaofei Liang;Tao Song;Xiaoqun Gong;R. Niu;Jin Chang
中科院分区：
文献类型：
--
作者：
Hanjie Wang;P. Zhao;Xiaofei Liang;Tao Song;Xiaoqun Gong;R. Niu;Jin Chang
设计和构建具有高稳定性和高效细胞摄取效率的载体是非常重要的。本研究成功地合成了聚乙二醇化十八烷基赖氨酸修饰壳聚糖(OQLCS)与胆固醇形成的具有较高尺寸稳定性和细胞摄取效率的新型聚合物脂质体(PLS)。与传统的脂质体(CLS；磷脂酰胆碱/胆固醇)相比，载钙黄绿素的PLS具有多层结构，粒径均匀(200 Nm)，钙黄绿素包封率高(约92%)。PLS可在不同温度(25℃、4℃、−20℃)和不同介质(去离子水、磷酸盐缓冲液和人血浆溶液)中保存4周，大小无明显变化。荧光分光光度分析和流式细胞仪分析表明，与CL相比，Zeta电位为正的PLS能促进MCF-7肿瘤细胞摄取钙黄绿素。这些数据表明，PLS可能为克服CLS的稳定性和提高其摄取效率提供一种新的方法。生物技术。比昂斯。2010；106：952-962。©2010 Wiley期刊，Inc.
The design and construction of delivery vectors with high stability and effective cellular uptake efficiency is very important. In this study, a novel polymeric liposomes (PLs) formed from PEGylated octadecyl‐quaternized lysine modified chitosan (OQLCS) and cholesterol with higher size stability and cellular uptake efficiency has been synthesized successfully. Compared to conventional liposomes (CLs; phosphatidyl choline/cholesterol), the calcein‐loaded PLs exhibited a multi‐lamellar structure with homogenous size diameter (200 nm) and high calcein encapsulation efficiency (about 92%). PLs could be stored at different temperature (25, 4, and −20°C) and different medium (deionized water, phosphate‐buffered saline, and human plasma solution) for up to 4 weeks without significant size change. The spectrophotometer fluorometry analysis and the flow cytometry analysis indicated that in comparison with CL, PLs with positive zeta potential facilitates the uptake of calcein by MCF‐7 tumor cells. The data suggests that PLs may provide a new method to overcome the stability and enhance the uptake efficiency of CLs. Biotechnol. Bioeng. 2010;106: 952–962. © 2010 Wiley Periodicals, Inc.