The projection structure of perfringolysin O (Clostridium perfringens theta-toxin).
The projection structure of perfringolysin O (Clostridium perfringens theta-toxin).
复制标题
产气荚膜梭菌溶血素 O（Clostridium perfringens theta-toxin）的投影结构。
作者：
A. Olofsson;H. Hebert;M. Thelestam
将溶细胞素 Perfringolysin O 应用于脂质层，并通过电子显微镜和图像处理分析获得的环形寡聚物。最终结果显示沿环外缘的周期性重复为 2.4 nm。不对称蛋白质单元对应于一个单体，横跨环从凸面到凹面。它显示出靠近外半径的清晰蛋白质峰和低聚物中间的密度较低。平均环内单体数量为50个，聚集体内半径约为15 nm。
The cytolysin Perfringolysin O was applied to lipid layers and the obtained ring-shaped oligomers analyzed by electron microscopy and image processing. The final result shows the periodic repeat of 2.4 nm along the outer rim of the ring. The asymmetric protein unit, corresponding to one monomer, spans the ring from the convex to the concave surface. It shows a clear protein peak close to the outer radius and less density in the middle of the oligomer. The number of monomers in the average ring is 50, and the inner radius of the aggregate is approximately 15 nm.