Optimization of the electrode drive pattern for imaging fascicular compound action potentials in peripheral nerve with fast neural electrical impedance tomography.
Optimization of the electrode drive pattern for imaging fascicular compound action potentials in peripheral nerve with fast neural electrical impedance tomography.
复制标题
通过快速神经电阻抗断层扫描优化周围神经束状复合动作电位成像的电极驱动模式。
DOI：
10.1088/1361-6579/ab54eb
复制
发表时间：
2019
影响因子：
3.2
通讯作者：
Ravagli E
中科院分区：
文献类型：
--
作者：
Ravagli E
本研究的主要目的是研究在使用外部圆柱形2× 14电极袖带的周围神经的快速神经EIT中，哪种注射模式导致束复合活动的最佳成像。具体而言，该研究解决了最佳注射模式和重建体积的最佳区域的图像fascicles.ApproachThe三种不同的测量协议功能（横向/纵向注射，驱动电极间距，参考配置）的效果的识别在模拟中使用现实的阻抗变化和噪声水平成像进行了研究。采用基于图像的度量来评估重建域上的重建质量。模拟建议的最佳电极寻址方案在大鼠坐骨周围神经胫骨和腓神经束（N= 3）上与MicroCT参考图像进行了体内验证。主要结果横向注入电流，电极间距为0.4个（0.100）和测量的单环参考，当在最接近参考的电极阵列的边缘上重建时导致最佳的整体定位。纵向注射方案导致重建图像的SNR较高，但定位较差。所有体内EIT记录具有统计学显著的阻抗变化（p< 0.05）。总体而言，束质中心（CoM）定位误差估计为141±56 µm（径向坐标为− 26±94 µm和5±29）。胫骨和腓骨CoMs的平均角度位置之间存在显著差异（p< 0.05）。SignificanceThis研究为读者提供了使用最有效的协议功能进行快速神经束复合活动EIT的建议。
ObjectiveThe main objective of this study was to investigate which injection pattern led to the best imaging of fascicular compound activity in fast neural EIT of peripheral nerve using an external cylindrical 2× 14-electrodes cuff. Specifically, the study addressed the identification of the optimal injection pattern and of the optimal region of the reconstructed volume to image fascicles.ApproachThe effect of three different measurement protocol features (transversal/longitudinal injection, drive electrode spacing, referencing configuration) over imaging was investigated in simulation with the use of realistic impedance changes and noise levels. Image-based metrics were employed to evaluate the quality of the reconstructions over the reconstruction domain. The optimal electrode addressing protocol suggested by the simulations was validated in vivo on the tibial and peroneal fascicles of rat sciatic peripheral nerves (N= 3) against MicroCT reference images.Main resultsInjecting current transversally, with spacing of⩾ 4 electrodes apart (⩾ 100) and single-ring referencing of measurements, led to the best overall localization when reconstructing on the edge of the electrode array closest to the reference. Longitudinal injection protocols led to a higher SNR of the reconstructed image but poorer localization. All in vivo EIT recordings had statistically significant impedance variations (p< 0.05). Overall, fascicle center-of-mass (CoM) localization error was estimated at 141±56 µm (− 26±94 µm and 5±29 in radial coordinates). Significant difference was found (p< 0.05) between mean angular location of the tibial and peroneal CoMs.SignificanceThis study gives the reader recommendations for performing fast neural EIT of fascicular compound activity using the most effective protocol features.