EXCESSIVE PRODUCTION OF REACTIVE OXYGEN METABOLITES BY INFLAMED COLON - ANALYSIS BY CHEMILUMINESCENCE PROBE
EXCESSIVE PRODUCTION OF REACTIVE OXYGEN METABOLITES BY INFLAMED COLON - ANALYSIS BY CHEMILUMINESCENCE PROBE
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DOI：
10.1016/0016-5085(92)91111-g
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发表时间：
1992-07-01
期刊：
影响因子：
29.4
通讯作者：
WINSHIP, D
中科院分区：
文献类型：
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作者：
KESHAVARZIAN, A;SEDGHI, S;WINSHIP, D
活性氧代谢物（ROM）参与炎症性疾病，并被认为有助于结肠炎中的组织损伤。为了确定发炎的结肠粘膜是否产生过量的ROM并鉴定ROM的可能来源和类型，使用化学发光探针估计大鼠和人的粘膜ROM。结肠内注射醋酸或腹腔内注射丝裂霉素C诱导大鼠结肠炎。大鼠的完整、发炎的结肠比正常结肠产生更多的超弱化学发光。两种大鼠模型的炎症粘膜刮片产生了显着更多的鲁米诺增强的化学发光。向培养基中添加过氧化氢酶（一种H2 O2清除剂）或叠氮化合物（一种髓过氧化物酶抑制剂）可显着降低炎症粘膜刮片的化学发光。吲哚美辛，一种抗氧化剂环氧合酶抑制剂，也降低化学发光，但MK-866，一种5-脂氧合酶抑制剂，没有影响。在结肠镜检查期间从溃疡性结肠炎患者获得的结肠活检标本也比正常结肠粘膜产生更多的过氧化氢酶可降解化学发光。这些数据表明，在人类和大鼠中，发炎的结肠粘膜都会产生过量的ROM，这可能会导致组织损伤。
Reactive oxygen metabolites (ROMs) are involved in inflammatory diseases and are postulated to contribute to tissue injury in colitis. To determine whether excessive ROMs are generated by inflamed colonic mucosa and to identify possible sources and type of ROMs, mucosal ROMs were estimated in rats and humans using a chemiluminescence probe. Colitis was induced in rats by intracolonic injection of acetic acid or intraperitoneal injection of mitomycin C. Intact, inflamed colon in rats produced more ultraweak chemiluminescence than normal colon. Inflamed mucosal scrapings from both rat models produced significantly more luminol-enhanced chemiluminescence. Addition of catalase, an H2O2scavenger, or azide, a myeloperoxidase inhibitor, into the media significantly decreased chemiluminescence from inflamed mucosal scrapings. Indomethacin, an antioxidant cyclo-oxygenase inhibitor, also decreased chemiluminescence, but MK-866, a 5-lipoxygenase inhibitor, had no effect. colonic biopsy specimens obtained during colonoscopy from patients with ulcerative colitis also produced more catalase-inhibitable chemiluminescence than normal colonic mucosa. These data indicate that excessive ROMs are produced by inflamed colonic mucosa in both humans and rats, which may contribute to tissue injury.